Characterization of a thermostable D-stereospecific alanine amidase from Brevibacillus borstelensis BCS-1

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dc.contributor.authorDae Heoun Back-
dc.contributor.authorSeok Joon Kwon-
dc.contributor.authorSeung Pyo Hong-
dc.contributor.authorMi Sun Kwak-
dc.contributor.authorMi Hwa Lee-
dc.contributor.authorJae Jun Song-
dc.contributor.authorSeung Goo Lee-
dc.contributor.authorK H Yoon-
dc.contributor.authorMoon Hee Sung-
dc.date.accessioned2017-04-19T08:59:44Z-
dc.date.available2017-04-19T08:59:44Z-
dc.date.issued2003-
dc.identifier.issn00992240-
dc.identifier.uri10.1128/AEM.69.2.980-986.2003ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6071-
dc.description.abstractA gene encoding a new thermostable D-stereospecific alanine amidase from the thermophile Brevibacillus borstelensis BCS-1 was cloned and sequenced. The molecular mass of the purified enzyme was estimated to be 199 kDa after gel filtration chromatography and about 30 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, indicating that the enzyme could be composed of a hexamer with identical subunits. The purified enzyme exhibited strong amidase activity towards D-amino acid-containing aromatic, aliphatic, and branched amino acid amides yet exhibited no enzyme activity towards L-amino acid amides, D-amino acid-containing peptides, and NH2-terminally protected amino acid amides. The optimum temperature and pH for the enzyme activity were 85°C and 9.0, respectively. The enzyme remained stable within a broad pH range from 7.0 to 10.0. The enzyme was inhibited by dithiothreitol, 2-mercaptoethanol, and EDTA yet was strongly activated by Co2+ and Mn2+. The kcat/Km for D-alaninamide was measured as 544.4 ± 5.5 mM-1 min-1 at 50°C with 1 mM Co2+.-
dc.publisherAmer Soc Microb-
dc.titleCharacterization of a thermostable D-stereospecific alanine amidase from Brevibacillus borstelensis BCS-1-
dc.title.alternativeCharacterization of a thermostable D-stereospecific alanine amidase from Brevibacillus borstelensis BCS-1-
dc.typeArticle-
dc.citation.titleApplied and Environmental Microbiology-
dc.citation.number2-
dc.citation.endPage986-
dc.citation.startPage980-
dc.citation.volume69-
dc.contributor.affiliatedAuthorJae Jun Song-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.alternativeName백대헌-
dc.contributor.alternativeName권석준-
dc.contributor.alternativeName홍승표-
dc.contributor.alternativeName곽미선-
dc.contributor.alternativeName이미화-
dc.contributor.alternativeName송재준-
dc.contributor.alternativeName이승구-
dc.contributor.alternativeName윤기홍-
dc.contributor.alternativeName성문희-
dc.identifier.bibliographicCitationApplied and Environmental Microbiology, vol. 69, no. 2, pp. 980-986-
dc.identifier.doi10.1128/AEM.69.2.980-986.2003-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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