An overexpression of chalcone reductase of Pueraria montana var. lobata alters biosynthesis of anthocyanin and 5'-deoxyflavonoids in transgenic tobacco

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Title
An overexpression of chalcone reductase of Pueraria montana var. lobata alters biosynthesis of anthocyanin and 5'-deoxyflavonoids in transgenic tobacco
Author(s)
Jae Youl Joung; G M Kasthuri; Ji Young Park; Won Jin Kang; Hyun Soon Kim; Bong Sik Yun; Hyouk Joung; Jae Heung Jeon
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 303, no. 1, pp. 326-331
Publication Year
2003
Abstract
We isolated the chalcone reductase (pl-chr) gene of Pueraria montana var. lobata by using a PCR strategy from cDNA pools of storage roots. A high level of expression of RNA was found in both stems and roots. The genomic Southern blot result suggests that pl-chr exists as a member of a small gene family. By introducing a pl-chr gene under the control of the 35S CaMV promoter into the pink-flowering Xanthi line of Nicotiana tabacum, the flower color was changed from pink to white-to-pink. The contents of anthocyanin in the flowers of the transgenic lines were dramatically decreased by 40%, but the total UV absorption compounds remained unchanged. The production of liquiritigenin in pl-chr overexpressed transgenic tobacco lines was confirmed by HPLC and MS analysis. The introduction of pl-chr gene provides a method to redirect the flavonoid pathway into 5′-deoxyflavonoid production in non-legume crops, in order to manipulate the phenylpropanoid pathway for isoflavonoid production.
Keyword
5′-DeoxyflavonoidsAnthocyaninChalcone reductaseIsoflavonoidPueraria montana var. lobataTransgenic tobacco
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0006-291X(03)00344-9
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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