Coexpression of BiP increased antithrombotic hirudin production in recombinant Saccharomyces cerevisiae

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Title
Coexpression of BiP increased antithrombotic hirudin production in recombinant Saccharomyces cerevisiae
Author(s)
M D Kim; K C Han; Hyun Ah Kang; Sang Ki Rhee; J H Seo
Bibliographic Citation
Journal of Biotechnology, vol. 101, no. 1, pp. 81-87
Publication Year
2003
Abstract
In order to increase a production level of antithrombotic hirudin, BiP was simultaneously expressed in recombinant Saccharomyces cerevisiae strains carrying ten and 15 copies of the hirudin expression cassette integrated in the chromosome. Coexpression of BiP greatly enhanced both cell growth and hirudin production in recombinant S. cerevisiae. Maximum hirudin concentration of 36 mg l-1 was obtained from batch culture of the ten copy-number transformant concomitantly harboring an episomal copy of the BiP gene under the control of the GAL1 promoter, which is corresponding to a 2.5-fold increase compared with the control strain carrying the genomic BiP gene only. The mean size of the recombinant yeast cells expressing the BiP gene remained at a relatively constant level compared with the control strains of which size increased after the onset of hirudin expression by the GAL10 promoter.
Keyword
BiPGAL10 promoterHirudinSaccharomyces cerevisiaeSecretion
ISSN
0168-1656
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0168-1656(02)00288-2
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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