Cloning, analysis, and expression of the gene for thermostable polyphosphate kinase of Thermus caldophilus GK24 and properties of the recombinant enzyme

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dc.contributor.authorH S Hoe-
dc.contributor.authorS K Lee-
dc.contributor.authorDae Sil Lee-
dc.contributor.authorS T Kwon-
dc.date.accessioned2017-04-19T08:59:45Z-
dc.date.available2017-04-19T08:59:45Z-
dc.date.issued2003-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6077-
dc.description.abstractThe gene encoding Thermus caldophilus GK24 polyphosphate kinase (Tca PPK) was cloned and sequenced. The gene contains an open reading frame encoding 608 amino acids with a calculated molecular mass of 69,850 Da. The deduced amino acid sequence of Tca PPK showed a 40% homology to Escherichia coli PPK, and 39% to Klebsiella aerogenes PPK. The Tca ppk gene was expressed under the control of the T7lac promoter on pET-22b(+) in E. coli and its enzyme was purified about 70-fold with 36% yield, following heating and HiTrap chelating HP column chromatography. The native enzyme was found to have an approximate molecular mass of 580,000 Da and consisted of eight subunits. The optimum pH and temperature of the enzyme were 5.5 and 70°C, respectively. A divalent cation was required for the enzyme activity, with Mg2+ being the most effective.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleCloning, analysis, and expression of the gene for thermostable polyphosphate kinase of Thermus caldophilus GK24 and properties of the recombinant enzyme-
dc.title.alternativeCloning, analysis, and expression of the gene for thermostable polyphosphate kinase of Thermus caldophilus GK24 and properties of the recombinant enzyme-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number1-
dc.citation.endPage145-
dc.citation.startPage139-
dc.citation.volume13-
dc.contributor.affiliatedAuthorDae Sil Lee-
dc.contributor.alternativeName허향숙-
dc.contributor.alternativeName이성경-
dc.contributor.alternativeName이대실-
dc.contributor.alternativeName권석태-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 13, no. 1, pp. 139-145-
dc.subject.keywordgene cloning-
dc.subject.keywordgene expression-
dc.subject.keywordpolyphosphate kinase-
dc.subject.keywordTca PPK-
dc.subject.keywordthermus caldophilus GK24-
dc.subject.localgene cloning-
dc.subject.localGene cloning-
dc.subject.localGene Expression-
dc.subject.localGene expression-
dc.subject.localgene expression-
dc.subject.localpolyphosphate kinase-
dc.subject.localTca PPK-
dc.subject.localthermus caldophilus GK-24-
dc.subject.localthermus caldosphilus Gk24-
dc.subject.localThermus caldophilus GK-24-
dc.subject.localThermus caldophilus GK24-
dc.subject.localthermus caldophilus GK24-
dc.description.journalClassY-
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