High expression of a human lactoferrin in transgenic tobacco cell cultures

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Title
High expression of a human lactoferrin in transgenic tobacco cell cultures
Author(s)
Sun Mee Choi; Ok Sun Lee; Suk Yoon KwonSang Soo Kwak; Dae Yeul Yu; Heang Soon Lee
Bibliographic Citation
Biotechnology Letters, vol. 25, no. 3, pp. 213-218
Publication Year
2003
Abstract
Transgenic Nicotiana tabacum cell lines were developed expressing the human lactoferrin gene driven by the oxidative stress-inducible peroxidase (SWPA2) promoter. Western blot analysis showed the accumulation of both the full-length human lactoferrin protein as well as a immuno-reactive truncated fragment. Accumulation of human lactoferrin as monitored by ELISA increased proportionally to cell growth and reached a maximal (up to 4.3% of total soluble proteins) at the stationary phase of growth. Protein extracts from transgenic tobacco cells exhibited antibacterial activity.
Keyword
High expression promoterHuman lactoferrinRecombinant proteinTransgenic plant cell culture
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1022341917735
Type
Article
Appears in Collections:
Division of Biomaterials Research > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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