A novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells

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dc.contributor.authorKee Yeun Kim-
dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorHeang Soon Lee-
dc.contributor.authorYun Kang Hur-
dc.contributor.authorJae Wook Bang-
dc.contributor.authorSang Soo Kwak-
dc.date.accessioned2017-04-19T08:59:46Z-
dc.date.available2017-04-19T08:59:46Z-
dc.date.issued2003-
dc.identifier.issn0167-4412-
dc.identifier.uri10.1023/A:1023045218815ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6083-
dc.description.abstractA strong oxidative stress-inducible peroxidase (POD) promoter was cloned from sweetpotato (Ipomoea batatas) and characterized in transgenic tobacco plants and cultured cells in terms of environmental stress. A POD genomic clone (referred to as SWPA2) consisted of 1824 bp of sequence upstream of the translation start site, two introns (743 bp and 97 bp), and a 1073 bp coding region. SWPA2 had previously been found to encode an anionic POD which was highly expressed in response to oxidative stress. The SWPA2 promoter contained several cis-element sequences implicated in oxidative stress such as GCN-4, AP-1, HSTF, SP-1 reported in animal cells and a plant specific G-box. Employing a transient expression assay in tobacco protoplasts, with five different 5′-deletion mutants of the SWPA2 promoter fused to the β-glucuronidase (GUS) reporter gene, the 1314 bp mutant deletion mutant showed about 30 times higher GUS expression than the CaMV 35S promoter. The expression of GUS activity in transgenic tobacco plants under the control of the -1314 SWPA2 promoter was strongly induced in response to environmental stresses including hydrogen peroxide, wounding and UV treatment. Furthermore, GUS activity in suspension cultures of transgenic cells derived from transgenic tobacco leaves containing the -1314 bp SWPA2 promoter-GUS fusion was strongly expressed after 15 days of subculture compared to other deletion mutants. We anticipate that the -1314 bp SWPA2 promoter will be biotechnologically useful for the development of transgenic plants with enhanced tolerance to environmental stress and particularly transgenic cell lines engineered to produce key pharmaceutical proteins.-
dc.publisherSpringer-
dc.titleA novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells-
dc.title.alternativeA novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells-
dc.typeArticle-
dc.citation.titlePlant Molecular Biology-
dc.citation.number6-
dc.citation.endPage838-
dc.citation.startPage831-
dc.citation.volume51-
dc.contributor.affiliatedAuthorKee Yeun Kim-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.affiliatedAuthorHeang Soon Lee-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.alternativeName김기연-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName이행순-
dc.contributor.alternativeName허윤강-
dc.contributor.alternativeName방재욱-
dc.contributor.alternativeName곽상수-
dc.identifier.bibliographicCitationPlant Molecular Biology, vol. 51, no. 6, pp. 831-838-
dc.identifier.doi10.1023/A:1023045218815-
dc.subject.keywordCultured cells-
dc.subject.keywordInducible promoter-
dc.subject.keywordIpomoea batatas-
dc.subject.keywordOxidative stress-
dc.subject.keywordPeroxidase-
dc.subject.keywordPromoter-deletion analysis-
dc.subject.localcultured cells-
dc.subject.localCultured cells-
dc.subject.localinducible promoter-
dc.subject.localInducible promoter-
dc.subject.localsweet potato-
dc.subject.localsweet potatoes-
dc.subject.localSweet potato (Ipomoea batatas L. Lam)-
dc.subject.localSweet potato (Ipomoea batatas)-
dc.subject.localSweet otato-
dc.subject.localsweet potato (Ipomoea batatas)-
dc.subject.localipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas)-
dc.subject.localsweetpotato-
dc.subject.localSweetpotato Ipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas L.)-
dc.subject.localSweetpotato-
dc.subject.localSweetpotato (Ipomoea batatas (L.) Lam)-
dc.subject.localSweet potato-
dc.subject.localIpomoea batatas-
dc.subject.localSweet potato (Ipomoea batatas (L.) Lam.)-
dc.subject.localOxidative stre-
dc.subject.localOxidative stress-
dc.subject.localOXIDATIVE STRESS-
dc.subject.localOxidative Stress-
dc.subject.localoxidative stress-
dc.subject.localperoxidase-
dc.subject.localPeroxidase-
dc.subject.localPromoter-deletion analysis-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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