Isolation of nitrogen-fixing bacteria from gramineous crops and measurement of nitrogenase activity = 벼과식물로부터 질소고정균의 분리와 Nitrogenase 활성 측정

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Title
Isolation of nitrogen-fixing bacteria from gramineous crops and measurement of nitrogenase activity = 벼과식물로부터 질소고정균의 분리와 Nitrogenase 활성 측정
Author(s)
E H Choi; S E Lee; K S Yoon; D K Kwon; J K Sohn; Seung Hwan Park; M S Han; S Y Ghim
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 31, no. 1, pp. 18-24
Publication Year
2003
Abstract
For researching nitrogen-fixing bacteria associated with gramineous crops, we collected growing roots of rices, wheats, oats, barleys, ryes, and maizes at 19 sites of southern Korean peninsula. Endophytes and free living bacteria were isolated from those crop roots. Sixty-three isolates were classified on the basis of different morphology, size, color, host of colony, and the 16S rDNAs sequence. The analyses of PCR amplification for nifH gene and nitrogenase activity assay, revealed that all isolates contained nitrogen-fixing abilities. In addition, most of them have cellulase activity which is one of the common features of endophytic bacteria from plant.
Keyword
16S rDNA sequencecellulasenitrogen-fixing bacterianitrogenase
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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