|dc.contributor.author||J G Kang||-|
|dc.contributor.author||J H Hur||-|
|dc.contributor.author||Bong Sik Yun||-|
|dc.contributor.author||Ick Dong Yoo||-|
|dc.contributor.author||K Y Kang||-|
|dc.description.abstract||An antagonistic fungus (AF5) was isolated from greenhouse soil. The fungus exhibited inhibitory activity against several phytopathogens in a dual culture on a potato dextrose agar medium, and was identified as Penicillium sp. based on its mycological characteristics. The antifungal compounds, sporogen AO-1 and p-hydroxybenzoic acid, were isolated from a culture broth of the Penicillium sp. AF5 and purified through solvent partition, silica gel column chromatography, and preparative TLC. Sporogen AO-1 showed strong activity either on mycelial growth (ED 50 = <40 ppm) of Phytophthora capsici, Pythium ultimum, and Rhizoctonia solani or zoospore germination of Phytophthora capsici. p-Hydroxybenzoic acid exhibited weak activity. Key words: Penicillium sp., antifungal activity, sporogen AO-1, p-hydroxybenzoic acid. The use of agricultural chemicals is essential for increasing food production. However, heavy dosage of pesticides, which has been applied in some cases to maintain a stable production of the crop, can bring about harmful side effects and resurgence of pests due to resistance to the pesticide. Biological control of disease is one of the means to reduce the potentially harmful fungicide use. 1) Recently, there has been increasing interest in research on pest management that exploits beneficial microorganisms to protect plants from phytopathogens. 2,3) Direct application of biocontrol agents, such as Aspergillus, 4) Trichoderma, 4,5) Chaetomium, 6) and Penicillium, 7) to plant pathogens has already been attempted. A large number of bioactive metabolites have also been isolated from various microbial sources, such as Actinomycetes, bacteria, and fungi. In addition, secondary metabolites of microbial origin have been discovered and commercialized. 8,9) These antibiotics exhibit little toxicity on the environment and plants. The genus Penicillium is ubiquitous in all environmental niches. The genus gained notice due to its ability to produce antibiotics, namely penicillin. 10) Recently, new antifungal antibiotics, such as BE-31405 11) and PF11643 12) , have been isolated from the Penicillium species. In the present study, we isolated a Penicillium sp. antagonistic to plant pathogenic fungi and identified two antifungal compounds from a culture broth of the isolate. Accordingly, taxonomy of the producing strain, fermentation, isolation, physico-chemical properties, and new antifungal activity of sporogen AO-1 and p-hydroxybenzoic acid are characterized in the current paper.||-|
|dc.title||Antifungal activity of sporogen AO-1 and p-hydroxybenzoic acid isolated from Penicillium sp. AF5||-|
|dc.title.alternative||Antifungal activity of sporogen AO-1 and p-hydroxybenzoic acid isolated from Penicillium sp. AF5||-|
|dc.citation.title||Journal of Applied Biological Chemistry||-|
|dc.identifier.bibliographicCitation||Journal of Applied Biological Chemistry, vol. 46, no. 1, pp. 33-37||-|
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