Characterization of B cell membrane receptors of polysaccharide isolated from the root of Acanthopanax koreanum

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dc.contributor.authorSang Bae Han-
dc.contributor.authorSong Kyu Park-
dc.contributor.authorH J Ahn-
dc.contributor.authorY D Yoon-
dc.contributor.authorY H Kim-
dc.contributor.authorJung Joon Lee-
dc.contributor.authorKi Hoon Lee-
dc.contributor.authorJae Sun Moon-
dc.contributor.authorHyoung-Chin Kim-
dc.contributor.authorHwan Mook Kim-
dc.date.accessioned2017-04-19T08:59:52Z-
dc.date.available2017-04-19T08:59:52Z-
dc.date.issued2003-
dc.identifier.issn15675769-
dc.identifier.uri10.1016/S1567-5769(03)00056-0ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6119-
dc.description.abstractWe investigated the immunomodulatory activity of polysaccharide isolated from the root of Acanthopanax koreanum (AK) at the cellular level. AK directly increased B cell proliferation and antibody production, but did not affect the expression of IL-2, IFN-γ or IL-4 by T cells, or T cell proliferation in vitro. Since AK cannot penetrate cells due to its large molecular mass, B cell activation may be caused by the surface binding of AK to B cell-specific receptors. The role of TLR4 as an AK receptor was shown by the fact that AK activity in B cells from C3H/HeJ mice, which are known to have a defective Toll-like receptor (TLR)-4, was found to be reduced compared with that in control cells from C3H/HeN mice. AK activity was also reduced by antibodies blocking TLR2, TLR4, CD19 or CD79b, but not by an antibody blocking CD38, which suggests AK receptor profiling in B cells. Two main differences between AK and lipopolysaccharide (LPS) were observed. First, LPS activity was inhibited by antibodies to either TLR2 or TLR4, but not by antibodies to CD19, CD79b or CD38. Another was that LPS-induced B cell proliferation was inhibited by polymyxin B (PMB), a specific inhibitor of LPS, whereas AK activity was not affected. Taken together, our results demonstrate that AK directly activates B cells, but not T cells, and suggest that AK has a broader receptor profile than LPS in B cells.-
dc.publisherElsevier-
dc.titleCharacterization of B cell membrane receptors of polysaccharide isolated from the root of Acanthopanax koreanum-
dc.title.alternativeCharacterization of B cell membrane receptors of polysaccharide isolated from the root of Acanthopanax koreanum-
dc.typeArticle-
dc.citation.titleInternational Immunopharmacology-
dc.citation.number5-
dc.citation.endPage691-
dc.citation.startPage683-
dc.citation.volume3-
dc.contributor.affiliatedAuthorKi Hoon Lee-
dc.contributor.affiliatedAuthorJae Sun Moon-
dc.contributor.affiliatedAuthorHyoung-Chin Kim-
dc.contributor.alternativeName한상배-
dc.contributor.alternativeName박성규-
dc.contributor.alternativeName안효정-
dc.contributor.alternativeName윤여대-
dc.contributor.alternativeName김영호-
dc.contributor.alternativeName이정준-
dc.contributor.alternativeName이기훈-
dc.contributor.alternativeName문제선-
dc.contributor.alternativeName김형진-
dc.contributor.alternativeName김환묵-
dc.identifier.bibliographicCitationInternational Immunopharmacology, vol. 3, no. 5, pp. 683-691-
dc.identifier.doi10.1016/S1567-5769(03)00056-0-
dc.subject.keywordAcanthopanax koreanum-
dc.subject.keywordB cell-
dc.subject.keywordPolysaccharide-
dc.subject.keywordReceptor-
dc.subject.keywordT cell-
dc.subject.localAcanthopanax koreanum-
dc.subject.localB cell-
dc.subject.localPolysaccharide-
dc.subject.localReceptor-
dc.subject.localT cell-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > 1. Journal Articles
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