Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding

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dc.contributor.authorSrinivas Ramisetti-
dc.contributor.authorHyun Ah Kang-
dc.contributor.authorSang Ki Rhee-
dc.contributor.authorChul Ho Kim-
dc.date.accessioned2017-04-19T08:59:53Z-
dc.date.available2017-04-19T08:59:53Z-
dc.date.issued2003-
dc.identifier.issn1226-8372-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6122-
dc.description.abstractThe artificial gene coding for anticoagulant hirudin was placed under the control of the GAL 10 promoter and expressed in the galactokinase-deficient strain (Δgal1) of Saccharomyces cerevisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L.-
dc.publisherSpringer-
dc.titleProduction of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding-
dc.title.alternativeProduction of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number3-
dc.citation.endPage186-
dc.citation.startPage183-
dc.citation.volume8-
dc.contributor.affiliatedAuthorSrinivas Ramisetti-
dc.contributor.affiliatedAuthorHyun Ah Kang-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.alternativeName스리니-
dc.contributor.alternativeName강현아-
dc.contributor.alternativeName이상기-
dc.contributor.alternativeName김철호-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 8, no. 3, pp. 183-186-
dc.identifier.doi10.1007/BF02935894-
dc.subject.keywordfed-batch fermentation-
dc.subject.keywordGAL promoter-
dc.subject.keywordgalactose-
dc.subject.keywordrecombinant hirudin-
dc.subject.keywordsaccharomyces cerevisiae-
dc.subject.localFed-batch fermentation-
dc.subject.localfed-batch fermentation-
dc.subject.localGAL promoter-
dc.subject.localgalactose-
dc.subject.localGalactose-
dc.subject.localRecombinant hirudin-
dc.subject.localrecombinant hirudin-
dc.subject.localSaccharomyces cerevisiae-
dc.subject.localsaccharomyces cerevisiae-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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