DC Field | Value | Language |
---|---|---|
dc.contributor.author | Srinivas Ramisetti | - |
dc.contributor.author | Hyun Ah Kang | - |
dc.contributor.author | Sang Ki Rhee | - |
dc.contributor.author | Chul Ho Kim | - |
dc.date.accessioned | 2017-04-19T08:59:53Z | - |
dc.date.available | 2017-04-19T08:59:53Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6122 | - |
dc.description.abstract | The artificial gene coding for anticoagulant hirudin was placed under the control of the GAL 10 promoter and expressed in the galactokinase-deficient strain (Δgal1) of Saccharomyces cerevisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L. | - |
dc.publisher | Springer | - |
dc.title | Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding | - |
dc.title.alternative | Production of recombinant hirudin in galactokinase-deficient Saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding | - |
dc.type | Article | - |
dc.citation.title | Biotechnology and Bioprocess Engineering | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 186 | - |
dc.citation.startPage | 183 | - |
dc.citation.volume | 8 | - |
dc.contributor.affiliatedAuthor | Srinivas Ramisetti | - |
dc.contributor.affiliatedAuthor | Hyun Ah Kang | - |
dc.contributor.affiliatedAuthor | Sang Ki Rhee | - |
dc.contributor.affiliatedAuthor | Chul Ho Kim | - |
dc.contributor.alternativeName | 스리니 | - |
dc.contributor.alternativeName | 강현아 | - |
dc.contributor.alternativeName | 이상기 | - |
dc.contributor.alternativeName | 김철호 | - |
dc.identifier.bibliographicCitation | Biotechnology and Bioprocess Engineering, vol. 8, no. 3, pp. 183-186 | - |
dc.identifier.doi | 10.1007/BF02935894 | - |
dc.subject.keyword | fed-batch fermentation | - |
dc.subject.keyword | GAL promoter | - |
dc.subject.keyword | galactose | - |
dc.subject.keyword | recombinant hirudin | - |
dc.subject.keyword | saccharomyces cerevisiae | - |
dc.subject.local | Fed-batch fermentation | - |
dc.subject.local | fed-batch fermentation | - |
dc.subject.local | GAL promoter | - |
dc.subject.local | galactose | - |
dc.subject.local | Galactose | - |
dc.subject.local | Recombinant hirudin | - |
dc.subject.local | recombinant hirudin | - |
dc.subject.local | Saccharomyces cerevisiae | - |
dc.subject.local | saccharomyces cerevisiae | - |
dc.description.journalClass | Y | - |
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