Enhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene

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dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorSun Mee Choi-
dc.contributor.authorYoung Ock Ahn-
dc.contributor.authorHeang Soon Lee-
dc.contributor.authorH B Lee-
dc.contributor.authorY M Park-
dc.contributor.authorSang Soo Kwak-
dc.date.accessioned2017-04-19T08:59:53Z-
dc.date.available2017-04-19T08:59:53Z-
dc.date.issued2003-
dc.identifier.issn0176-1617-
dc.identifier.uri10.1078/0176-1617-00926ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6125-
dc.description.abstractTo analyze the physiological role of dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzing the reduction of DHA to ascorbate in environmental stress adaptation, T1 transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants expressing a human DHAR gene in chloroplasts were biochemically characterized and tested for responses to various stresses. Fully expanded leaves of transgenic plants had about 2.29 times higher DHAR activity (units/g fresh wt) than non-transgenic (NT) plants. Interestingly, transgenic plants also showed a 1.43 times higher glutathione reductase activity than NT plants. As a result, the ratio of AsA/DHA was changed from 0.21 to 0.48, even though total ascorbate content was not significantly changed. When tobacco leaf discs were subjected to methyl viologen (MV) at 5 μmol/L and hydrogen peroxide (H2O2) at 200 mmol/L, transgenic plants showed about a 40% and 25% reduction in membrane damage relative to NT plants, respectively. Furthermore, transgenic seedlings showed enhanced tolerance to low temperature (15°C) and NaCl (100 mmol/L) compared to NT plants. These results suggest that a human derived DHAR properly works for the protection against oxidative stress in plants.-
dc.publisherElsevier-
dc.titleEnhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene-
dc.title.alternativeEnhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene-
dc.typeArticle-
dc.citation.titleJournal of Plant Physiology-
dc.citation.number4-
dc.citation.endPage353-
dc.citation.startPage347-
dc.citation.volume160-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.affiliatedAuthorSun Mee Choi-
dc.contributor.affiliatedAuthorYoung Ock Ahn-
dc.contributor.affiliatedAuthorHeang Soon Lee-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName최선미-
dc.contributor.alternativeName안영옥-
dc.contributor.alternativeName이행순-
dc.contributor.alternativeName이해복-
dc.contributor.alternativeName박용목-
dc.contributor.alternativeName곽상수-
dc.identifier.bibliographicCitationJournal of Plant Physiology, vol. 160, no. 4, pp. 347-353-
dc.identifier.doi10.1078/0176-1617-00926-
dc.subject.keywordAscorbic acid-
dc.subject.keywordDehydroascorbate reductase-
dc.subject.keywordGlutathione-
dc.subject.keywordMethyl viologen-
dc.subject.keywordOxidative stress-
dc.subject.keywordTransgenic tobacco plant-
dc.subject.localascorbic acid-
dc.subject.localAscorbic acid-
dc.subject.localAscorbic acid (vitamin C)-
dc.subject.localDehydroascorbate reductase-
dc.subject.localdehydroascorbate reductase-
dc.subject.localglutathione-
dc.subject.localGlutathione-
dc.subject.localmethyl viologen-
dc.subject.localMethyl viologen-
dc.subject.localOxidative stre-
dc.subject.localOxidative stress-
dc.subject.localOXIDATIVE STRESS-
dc.subject.localOxidative Stress-
dc.subject.localoxidative stress-
dc.subject.localtransgenic tobacco plant-
dc.subject.localTransgenic tobacco plant-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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