DC Field | Value | Language |
---|---|---|
dc.contributor.author | Suk Yoon Kwon | - |
dc.contributor.author | Sun Mee Choi | - |
dc.contributor.author | Young Ock Ahn | - |
dc.contributor.author | Heang Soon Lee | - |
dc.contributor.author | H B Lee | - |
dc.contributor.author | Y M Park | - |
dc.contributor.author | Sang Soo Kwak | - |
dc.date.accessioned | 2017-04-19T08:59:53Z | - |
dc.date.available | 2017-04-19T08:59:53Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0176-1617 | - |
dc.identifier.uri | 10.1078/0176-1617-00926 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6125 | - |
dc.description.abstract | To analyze the physiological role of dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzing the reduction of DHA to ascorbate in environmental stress adaptation, T1 transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants expressing a human DHAR gene in chloroplasts were biochemically characterized and tested for responses to various stresses. Fully expanded leaves of transgenic plants had about 2.29 times higher DHAR activity (units/g fresh wt) than non-transgenic (NT) plants. Interestingly, transgenic plants also showed a 1.43 times higher glutathione reductase activity than NT plants. As a result, the ratio of AsA/DHA was changed from 0.21 to 0.48, even though total ascorbate content was not significantly changed. When tobacco leaf discs were subjected to methyl viologen (MV) at 5 μmol/L and hydrogen peroxide (H2O2) at 200 mmol/L, transgenic plants showed about a 40% and 25% reduction in membrane damage relative to NT plants, respectively. Furthermore, transgenic seedlings showed enhanced tolerance to low temperature (15°C) and NaCl (100 mmol/L) compared to NT plants. These results suggest that a human derived DHAR properly works for the protection against oxidative stress in plants. | - |
dc.publisher | Elsevier | - |
dc.title | Enhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene | - |
dc.title.alternative | Enhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene | - |
dc.type | Article | - |
dc.citation.title | Journal of Plant Physiology | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 353 | - |
dc.citation.startPage | 347 | - |
dc.citation.volume | 160 | - |
dc.contributor.affiliatedAuthor | Suk Yoon Kwon | - |
dc.contributor.affiliatedAuthor | Sun Mee Choi | - |
dc.contributor.affiliatedAuthor | Young Ock Ahn | - |
dc.contributor.affiliatedAuthor | Heang Soon Lee | - |
dc.contributor.affiliatedAuthor | Sang Soo Kwak | - |
dc.contributor.alternativeName | 권석윤 | - |
dc.contributor.alternativeName | 최선미 | - |
dc.contributor.alternativeName | 안영옥 | - |
dc.contributor.alternativeName | 이행순 | - |
dc.contributor.alternativeName | 이해복 | - |
dc.contributor.alternativeName | 박용목 | - |
dc.contributor.alternativeName | 곽상수 | - |
dc.identifier.bibliographicCitation | Journal of Plant Physiology, vol. 160, no. 4, pp. 347-353 | - |
dc.identifier.doi | 10.1078/0176-1617-00926 | - |
dc.subject.keyword | Ascorbic acid | - |
dc.subject.keyword | Dehydroascorbate reductase | - |
dc.subject.keyword | Glutathione | - |
dc.subject.keyword | Methyl viologen | - |
dc.subject.keyword | Oxidative stress | - |
dc.subject.keyword | Transgenic tobacco plant | - |
dc.subject.local | ascorbic acid | - |
dc.subject.local | Ascorbic acid | - |
dc.subject.local | Ascorbic acid (vitamin C) | - |
dc.subject.local | Dehydroascorbate reductase | - |
dc.subject.local | dehydroascorbate reductase | - |
dc.subject.local | glutathione | - |
dc.subject.local | Glutathione | - |
dc.subject.local | methyl viologen | - |
dc.subject.local | Methyl viologen | - |
dc.subject.local | Oxidative stre | - |
dc.subject.local | Oxidative stress | - |
dc.subject.local | OXIDATIVE STRESS | - |
dc.subject.local | Oxidative Stress | - |
dc.subject.local | oxidative stress | - |
dc.subject.local | transgenic tobacco plant | - |
dc.subject.local | Transgenic tobacco plant | - |
dc.description.journalClass | Y | - |
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