Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells

Cited 2 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorYong Hee Lee-
dc.contributor.authorH E Broxmeyer-
dc.contributor.authorC Mantel-
dc.contributor.authorH J Kwon-
dc.contributor.authorJae Wha Kim-
dc.contributor.authorJin Sook Kim-
dc.contributor.authorDur Han Kwon-
dc.contributor.authorIn Seong Choe-
dc.date.accessioned2017-04-19T08:59:56Z-
dc.date.available2017-04-19T08:59:56Z-
dc.date.issued2003-
dc.identifier.issnI000-0028-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6132-
dc.description.abstractStem cell factor (SCF) is an early-acting cytokine inducing proliferative synergy with other cytokines in hematopoietic cells. We earlier showed that p21 was synergistically induced in SCF synergy and the p44/42 MAPK pathway was essential for the transcriptional control of p21. SCF synergy accompanies protein synthesis. p70S6K implicated in translational control in many other systems has not been shown in SCF synergy induced system. GM-CSF dependent human cell line MO7e was stimulated with GM-CSF with SCF, and investigated activation of p70S6K by using phospho-specific antibody. A possible contribution of p70S6K to SCF synergy was examined by measuring p21 induction as a model system. p70S6K was slightly activated by GM-CSF alone and markedly activated by SCF alone. Combined stimulation with these two cytokines synergistically activated p70S6K resulting in persistent activation. Addition of the pathway-specific inhibitors for PI3K or FRAP/TOR, two upstream pathways of p70S6K resulted in abolishment of p70S6K phosphorylation and also significant reduction of p21 protein level. These data suggest that synergistically activated p70S6K by GM-CSF plus SCF involves, at least in part, protein translational control including regulation of p21 protein.-
dc.publisherSpringer-Nature Pub Group-
dc.titleSynergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells-
dc.title.alternativeSynergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells-
dc.typeArticle-
dc.citation.titleExperimental and Molecular Medicine-
dc.citation.number3-
dc.citation.endPage226-
dc.citation.startPage222-
dc.citation.volume35-
dc.contributor.affiliatedAuthorYong Hee Lee-
dc.contributor.affiliatedAuthorJae Wha Kim-
dc.contributor.affiliatedAuthorJin Sook Kim-
dc.contributor.affiliatedAuthorDur Han Kwon-
dc.contributor.affiliatedAuthorIn Seong Choe-
dc.contributor.alternativeName이영희-
dc.contributor.alternativeNameBroxmeyer-
dc.contributor.alternativeNameMantel-
dc.contributor.alternativeName권형주-
dc.contributor.alternativeName김재화-
dc.contributor.alternativeName김진숙-
dc.contributor.alternativeName권두한-
dc.contributor.alternativeName최인성-
dc.identifier.bibliographicCitationExperimental and Molecular Medicine, vol. 35, no. 3, pp. 222-226-
dc.identifier.doi10.1038/emm.2003.30-
dc.subject.keywordhematopoietic progenitors-
dc.subject.keywordp70S6K-
dc.subject.keywordSCF synergy-
dc.subject.keywordtranslational control-
dc.subject.localhematopoietic progenitors-
dc.subject.localp70S6K-
dc.subject.localSCF synergy-
dc.subject.localtranslational control-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.