Peroxiredoxin II is essential for sustaining life span of erythrocytes in mice

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Title
Peroxiredoxin II is essential for sustaining life span of erythrocytes in mice
Author(s)
Tae Hoon Lee; Sun-Uk Kim; Seong Lan Yu; Sue Hee Kim; D S Park; H B Moon; S H Dho; Ki Sun Kwon; Hyun Jung Kwon; Y H Han; Sang Kyun Jeong; S W Kang; H S Shim; Kyung Kwang Lee; S G Rhee; Dae Yeul Yu
Bibliographic Citation
Blood, vol. 101, no. 12, pp. 5033-5038
Publication Year
2003
Abstract
Peroxiredoxins (Prxs) are a family of antioxidant proteins that reduce peroxide levels by using reducing agents such as thioredoxin. These proteins were characterized to have a number of cellular functions, including cell proliferation and differentiation and protection of specific proteins from oxidative damage. However, the physiological roles of the peroxiredoxins have not been determined. To clarify the physiological relevance of this protein type, we generated a mouse model deficient in Prx II, which is abundantly expressed in all types of cells. The Prx II-/- mice were healthy in appearance and fertile. However, they had splenomegaly caused by the congestion of red pulp with hemosiderin accumulation. Heinz bodies were detected in their peripheral blood, and morphologically abnormal cells were elevated in the dense red blood cell (RBC) fractions, which contained markedly higher levels of reactive oxygen species (ROS). The Prx II-/- mice had significantly decreased hematocrit levels, but increased reticulocyte counts and erythropoietin levels, indicative of a compensatory action to maintain hematologic homeostasis in the mice. In addition, a labeling experiment with the thiol-modifying reagent biotinylated iodoacetamide (BIAM) in Prx II-/- mice revealed that a variety of RBC proteins were highly oxidized. Our results suggest that Prx II-/- mice have hemolytic anemia and that Prx II plays a major role in protecting RBCs from oxidative stress in mice.
ISSN
0006-4971
Publisher
Amer Soc Hematology
DOI
http://dx.doi.org/10.1182/blood-2002-08-2548
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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