Functional identification of rub52 gene involved in the biosynthesis of rubradirin

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Title
Functional identification of rub52 gene involved in the biosynthesis of rubradirin
Author(s)
J Maharjan; K Liou; H C Lee; C G Kim; Jung Joon Lee; J C Yoo; J K Sohng
Bibliographic Citation
Biotechnology Letters, vol. 25, no. 11, pp. 909-915
Publication Year
2003
Abstract
An open reading frame, rub52, has been identified as a gene encoding thymidine diphospho-glucose 2,3-dehydratase by sequence analysis of the rubradirin biosynthetic gene cluster of Streptomyces achromogenes var. rubradiris NRRL3061. The gene codes for a protein consisting of 458 amino acids with calculated molecular mass of 50 862 Da. The gene was amplified and heterologously expressed in Escherichia coli as a soluble His-tagged fusion protein. C-2 deoxygenation functionality of thymidine diphospho-4-keto-6-deoxyglucose was assigned to the rub52 gene product from in vitro enzyme assay.
Keyword
Streptomyces achromogensD-rubranitroseDeoxygenationHeterologous expression
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1024075902143
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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