High-level expression of human glycosyltransferases in insect cells as biochemically active form

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dc.contributor.authorH G Kim-
dc.contributor.authorS M Yang-
dc.contributor.authorY C Lee-
dc.contributor.authorSu Il Do-
dc.contributor.authorI S Chung-
dc.contributor.authorJ M Yang-
dc.date.accessioned2017-04-19T08:59:58Z-
dc.date.available2017-04-19T08:59:58Z-
dc.date.issued2003-
dc.identifier.issn0006-291X-
dc.identifier.uri10.1016/S0006-291X(03)00795-2ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6145-
dc.description.abstractcDNAs, encoding human β1,4-galactosyltransferase (hGalT I, EC 2.4.1.22), human Galβ1,3(4)-GlcNAc α2,3-sialyltransferase (hST3GalIII, EC 2.4.99), and human Galβ1,4-GlcNAc α2,6-sialyltransferase (hST6Gal I, EC 2.4.99.1), were cloned from human cell lines. In order to express these glycosyltransferases as secreted form in insect cells, cDNAs were inserted into a novel baculovirus transfer vector equipped with the mouse IgM signal peptide and IgG binding domain of the Staphylococcus aureus protein A as an N-terminal fusion partner. About 14mg hGalT I, 8mg hST3GalIII, and 6.4mg hST6Gal I were purified from 1liter of recombinant baculovirus infected insect cell culture media. The specific activities of recombinant hGalT I and hST6Gal I were determined as 0.65 and 1.6U/mg protein, respectively. These results indicated that the recombinant hGalT I and hST6Gal I retained enzyme activities at similar level to those of the authentic one although they were fused with the IgG binding domain at the N-terminus. Taken together, the mouse IgM signal peptide and IgG binding domain of the protein A could be efficiently used as an N-terminus fusion partner for the over-expression of heterologous proteins in insect cells.-
dc.publisherElsevier-
dc.titleHigh-level expression of human glycosyltransferases in insect cells as biochemically active form-
dc.title.alternativeHigh-level expression of human glycosyltransferases in insect cells as biochemically active form-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number3-
dc.citation.endPage493-
dc.citation.startPage488-
dc.citation.volume305-
dc.contributor.affiliatedAuthorSu Il Do-
dc.contributor.alternativeName김형구-
dc.contributor.alternativeName양승모-
dc.contributor.alternativeName이영춘-
dc.contributor.alternativeName도수일-
dc.contributor.alternativeName정인식-
dc.contributor.alternativeName양재명-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 305, no. 3, pp. 488-493-
dc.identifier.doi10.1016/S0006-291X(03)00795-2-
dc.subject.keywordGalactosyltransferase-
dc.subject.keywordHeterologous expression-
dc.subject.keywordIgM signal peptide-
dc.subject.keywordInsect cell-
dc.subject.keywordProtein A-
dc.subject.keywordSialyltransferase-
dc.subject.localGalactosyltransferase-
dc.subject.localheterologous expression-
dc.subject.localHeterologous expression-
dc.subject.localIgM signal peptide-
dc.subject.localInsect cells-
dc.subject.localinsect cell-
dc.subject.localInsect cell-
dc.subject.localProtein A-
dc.subject.localSialyltransferase-
dc.subject.localsialyltransferase-
dc.description.journalClassY-
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