DC Field | Value | Language |
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dc.contributor.author | Suk Weon Kim | - |
dc.contributor.author | D S In | - |
dc.contributor.author | Tae Jin Kim | - |
dc.contributor.author | Jang Ryol Liu | - |
dc.date.accessioned | 2017-04-19T08:59:58Z | - |
dc.date.available | 2017-04-19T08:59:58Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0167-6857 | - |
dc.identifier.uri | 10.1023/A:1023997627578 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6146 | - |
dc.description.abstract | Culture conditions are described for high frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis Max. Petiole explants formed embryogenic calluses at a frequency of 53% when cultured on B5 medium supplemented with 13.6 μM 2,4-dichlorophenoxyacetic acid (2,4-D) alone. Leaf explants formed embryogenic calluses at a frequency of 21% when cultured at a combination of 4.52 μM 2,4-D and 2.22 μM 6-benzyladenine. Cell suspension cultures were established with petiole-derived embryogenic calluses using liquid B5 medium with 4.52 μM 2,4-D. Upon plating onto B5 basal medium, cell suspension cultures produced numerous somatic embryos, which then developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse. | - |
dc.publisher | Springer | - |
dc.title | High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis | - |
dc.title.alternative | High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis | - |
dc.type | Article | - |
dc.citation.title | Plant Cell Tissue and Organ Culture | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 167 | - |
dc.citation.startPage | 163 | - |
dc.citation.volume | 74 | - |
dc.contributor.affiliatedAuthor | Suk Weon Kim | - |
dc.contributor.affiliatedAuthor | Tae Jin Kim | - |
dc.contributor.affiliatedAuthor | Jang Ryol Liu | - |
dc.contributor.alternativeName | 김석원 | - |
dc.contributor.alternativeName | 인동수 | - |
dc.contributor.alternativeName | 김태진 | - |
dc.contributor.alternativeName | 유장렬 | - |
dc.identifier.bibliographicCitation | Plant Cell Tissue and Organ Culture, vol. 74, no. 2, pp. 163-167 | - |
dc.identifier.doi | 10.1023/A:1023997627578 | - |
dc.subject.keyword | Medicinal plant | - |
dc.subject.keyword | Papaveracea | - |
dc.subject.keyword | Somatic embryo | - |
dc.subject.local | medicinal plant | - |
dc.subject.local | medicinal plants | - |
dc.subject.local | Medicinal plant | - |
dc.subject.local | Medicinal plants | - |
dc.subject.local | Papaveracea | - |
dc.subject.local | Papaveraceae | - |
dc.subject.local | Somatic embryo | - |
dc.subject.local | somatic embryo | - |
dc.subject.local | somatic embryos | - |
dc.description.journalClass | Y | - |
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