Dexamethasone reverses TGF-β-mediated inhibition of primary rat preadipocyte differentiation

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Title
Dexamethasone reverses TGF-β-mediated inhibition of primary rat preadipocyte differentiation
Author(s)
Sun Mi Shin; Kun Yong Kim; Jae Kwang Kim; Suk Ran YoonIn Pyo Choi; Young Yang
Bibliographic Citation
FEBS Letters, vol. 543, no. 1, pp. 25-30
Publication Year
2003
Abstract
Dexamethasone and transforming growth factor-β (TGF-β) show contrary effects on differentiation of adipocytes. Dexamethasone stimulates adipocyte differentiation whereas TGF-β inhibits it. In the present study, we investigated whether dexamethasone could reverse the TGF-β-mediated inhibition of preadipocyte differentiation. Primary rat preadipocytes, obtained from Sprague-Dawley rats, were pretreated with dexamethasone in the presence or absence of TGF-β, prior to the induction of differentiation. Co-treatment of dexamethasone and TGF-β before inducing differentiation reversed the TGF-β-mediated inhibition of preadipocyte differentiation. In order to elucidate the mechanism by which dexamethasone reversed the effect of TGF-β on the inhibition of preadipocyte differentiation, the expression of CCAAT/enhancer binding protein-α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ) was examined. Dexamethasone increased C/EBPα and PPARγ expression in the absence of TGF-β and also recovered the TGF-β-mediated suppression of C/EBPα expression in preadipocytes. Its effect was sustained in differentiated adipocytes as well. However, those effects were not observed in 3T3-L1 preadipocytes or differentiated adipocytes. These results indicate that dexamethasone reverses the TGF-β-mediated suppression of adipocyte differentiation by regulating the expression of C/EBPα and PPARγ, which is dependent on the cellular context.
Keyword
CCAAT/enhancer binding protein-αDexamethasonePeroxisome proliferator-activated receptor γRat preadipocyteTransforming growth factor-β
ISSN
0014-5793
Publisher
Wiley
DOI
http://dx.doi.org/10.1016/S0014-5793(03)00371-5
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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