Drug-induced haploinsufficiency of fission yeast provides a powerful tool for identification of drug targets

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Title
Drug-induced haploinsufficiency of fission yeast provides a powerful tool for identification of drug targets
Author(s)
Jo Young Park; Young Joo Jang; S J You; Young Sook Kil; Eun Jung Kang; Jee Hee Ahn; Young Kwon Ryoo; Min Youn Lee; S Y Park; H J Lee; J Y Kim; S H Kim; W S Yang; K B Song; H M Park; Y J Chung; H B Kim; Kwang Lae Hoe; Kyung Sook ChungDong Uk Kim; Hyang Sook Yoo; Mi Sun Won
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 13, no. 2, pp. 317-320
Publication Year
2003
Abstract
Genome-wide systematic deletion mutants were generated using a PCR-based targeted mutagenesis of Schizosaccharomyces pombe. In a drug-sensitivity assay using thiabendazole (TBZ), an inhibitor of microtubule assembly, a heterozygous nda2 mutant (nda2+/nda2-), deleting one copy of nda2 encoding the microtubule subunit alpha 1 demonstrated a distinct sensitivity to TBZ, indicating TBZ-induced haploinsufficiency. This result suggests that profiling drug-induced haploinsufficiency can be exploited to identify target genes for drugs and discover new drugs.
Keyword
drug targethaploinsufficiencyPCR-based targeted mutagenesisschizosaccharomyces pombe
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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