Control of direct and indirect somatic embryogenesis by exogenous growth regulators in immature zygotic embryo cultures of rose

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Title
Control of direct and indirect somatic embryogenesis by exogenous growth regulators in immature zygotic embryo cultures of rose
Author(s)
Suk Weon Kim; Seung Chul Oh; Jang Ryol Liu
Bibliographic Citation
Plant Cell Tissue and Organ Culture, vol. 74, no. 1, pp. 61-66
Publication Year
2003
Abstract
Immature zygotic embryos of rose (Rosa hybrida L.; cv. Sumpath) did not form somatic embryos or embryogenic calluses when cultured on half-strength Murashige and Skoog's medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. However, the zygotic embryos produced somatic embryos without an intervening callus phase at a frequency of 27.3% on medium with 4.44 μM 6-benzyladenine (BA) alone. Immature zygotic embryos formed embryogenic calluses at a frequency of 25% on medium with a combination of 1.36 μM 2,4-D and 4.44 μM BA. Upon transfer to medium without growth regulators, embryogenic calluses produced numerous somatic embryos that subsequently developed into plantlets. Somatic embryos were induced directly from immature zygotic embryos, or indirectly via an intervening callus phase, by manipulating the exogenous growth regulators. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.
Keyword
Embryogenic callusImmature zygotic embryoPlant regenerationRosa hybrida
ISSN
0167-6857
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1023355729046
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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