Characterization of an extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Streptomyces sp. KJ-72

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dc.contributor.authorH J Kim-
dc.contributor.authorDo Young Kim-
dc.contributor.authorJ S Nam-
dc.contributor.authorKyung Sook Bae-
dc.contributor.authorY H Rhee-
dc.date.accessioned2017-04-19T09:00:02Z-
dc.date.available2017-04-19T09:00:02Z-
dc.date.issued2003-
dc.identifier.issn0003-6072-
dc.identifier.uri10.1023/A:1023395527073ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6168-
dc.description.abstractA bacterial strain capable of degrading medium-chain-length polyhydroxyalkanoates (MCL-PHAs) was isolated from a soil sample. This organism, which was identified as Streptomyces sp. KJ-72, secreted MCL-PHA depolymerase into the culture fluid only when it was cultivated on MCL-PHAs. The extracellular MCL-PHA depolymerase of the organism was purified to electrophoretic homogeneity by ion exchange column chromatography and gel filtration. The enzyme consisted of a monomeric subunit having a molecular mass of 27.1 kDa and isoelectric point of 4.7. The maximum activity was observed at pH 8.7 and 50 °C. The enzyme was sensitive to N-bromosuccinimide and acetic anhydride, indicating the presence of tryptophan and lysine residues in the catalytic domain. The enzyme was able to hydrolyze various chain-length p-nitrophenyl esters of fatty acids and polycaprolactone as well as various types of MCL-PHAs. However, lipase activity of the enzyme was not detected. The main hydrolysis product of poly(3-hydroxyheptanoate) was identified to be the dimer of 3-hydroxyheptanoate.-
dc.publisherSpringer-
dc.titleCharacterization of an extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Streptomyces sp. KJ-72-
dc.title.alternativeCharacterization of an extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Streptomyces sp. KJ-72-
dc.typeArticle-
dc.citation.titleAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology-
dc.citation.number2-
dc.citation.endPage189-
dc.citation.startPage183-
dc.citation.volume83-
dc.contributor.affiliatedAuthorDo Young Kim-
dc.contributor.affiliatedAuthorKyung Sook Bae-
dc.contributor.alternativeName김화정-
dc.contributor.alternativeName김도영-
dc.contributor.alternativeName남진식-
dc.contributor.alternativeName배경숙-
dc.contributor.alternativeName이영하-
dc.identifier.bibliographicCitationAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, vol. 83, no. 2, pp. 183-189-
dc.identifier.doi10.1023/A:1023395527073-
dc.subject.keywordMedium-chain-length polyhydroxyalkanoate-
dc.subject.keywordPolyhydroxyalkanoate depolymerase-
dc.subject.keywordStreptomyces sp. KJ-72-
dc.subject.localMedium-chain-length polyhydroxyalkanoates-
dc.subject.localMedium-chain-length polyhydroxyalkanoate-
dc.subject.localPolyhydroxyalkanoate depolymerase-
dc.subject.localStreptomyces sp. KJ-72-
dc.description.journalClassY-
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Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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