A Novel approach to isolation and functional characterization of genomic DNA sequences from the methylotrophic yeast Hansenula polymorpha

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Title
A Novel approach to isolation and functional characterization of genomic DNA sequences from the methylotrophic yeast Hansenula polymorpha
Author(s)
M O Agaphonov; A V Deev; S Y Kim; Jung Hoon Sohn; Eui Sung Choi; M D Ter-Avanesyan
Bibliographic Citation
Molecular Biology, vol. 37, no. 1, pp. 74-80
Publication Year
2003
Abstract
A novel approach to isolation and functional characterization of the Hansenula polymorpha genes basing on the use of two strains of different origin is described. One of these strains is better suited for the isolation of genomic DNA fragments, while the other is preferable for their functional analysis. Thirty three genomic sequences governing expression of a reporter protein have been isolated. Analysis of the sequence encoding a homolog of the Saccharomyces cerevisiae cofilin revealed two introns. Another isolated DNA fragment encoded a homolog of the S. cerevisiae Vps10p. Disruption of the corresponding gene resulted in secretion of a vacuolar protein, carboxypeptidase Y, into the culture medium.
Keyword
Hansenula polymorphaIntronProtein secretionRecombinationSplicingVacuole
ISSN
0026-8933
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1022336914110
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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