Plant regeneration of rose (Rosa hybridia) from embryogenic cell-derived protoplasts

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dc.contributor.authorSuk Weon Kim-
dc.contributor.authorSeung Chul Oh-
dc.contributor.authorD S In-
dc.contributor.authorJang Ryol Liu-
dc.date.accessioned2017-04-19T09:00:07Z-
dc.date.available2017-04-19T09:00:07Z-
dc.date.issued2003-
dc.identifier.issn0167-6857-
dc.identifier.uri10.1023/A:1022693605436ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6176-
dc.description.abstractCulture conditions are described for sustained cell division and plant regeneration from protoplasts of rose (Rosa hybrida L. 'Sumpath'). Protoplasts were enzymatically isolated from 2-week-old embryogenic cell suspension cultures. Freshly isolated protoplasts were plated as a thin layer onto protoplast culture medium (half-strength Murashige and Skoog's medium containing 60 g l-1 myo-inositol, 4.4 μM BA, and 1.4 μM 2,4-D) at a density of 5×104 protoplasts ml-1. The plating efficiency reached 3.9% after 2 weeks of culture. However, few protoplasts underwent cell division when cultured in protoplast culture medium in which 60 g l-1 myo-inositol was replaced with the same osmolarity of 90 g l-1 mannitol, indicating that myo-inositol is essential for sustained cell division of protoplasts. Colonies were formed after 8 weeks of culture at a frequency of 0.2%. Colonies were then transferred to colony culture medium (0.4% Gelrite-solidified protoplast culture medium) and maintained by subculturing at 4-week intervals to form embryogenic calluses. Upon transfer to half-strength MS basal medium, embryogenic calluses gave rise to numerous somatic embryos. Somatic embryos were transferred to half-strength MS basal medium containing 48 mg l-1 ferric ethylenediamine di-(o-hydroxyphenylacetate), where they subsequently developed into plantlets at a frequency of 30.9%. The plantlets had the same chromosome number of 2n=3x=21 as the source plant. They were successfully transplanted to potting soil and grown to maturity in a greenhouse.-
dc.publisherSpringer-
dc.titlePlant regeneration of rose (Rosa hybridia) from embryogenic cell-derived protoplasts-
dc.title.alternativePlant regeneration of rose (Rosa hybridia) from embryogenic cell-derived protoplasts-
dc.typeArticle-
dc.citation.titlePlant Cell Tissue and Organ Culture-
dc.citation.number1-
dc.citation.endPage19-
dc.citation.startPage15-
dc.citation.volume73-
dc.contributor.affiliatedAuthorSuk Weon Kim-
dc.contributor.affiliatedAuthorSeung Chul Oh-
dc.contributor.affiliatedAuthorJang Ryol Liu-
dc.contributor.alternativeName김석원-
dc.contributor.alternativeName오승철-
dc.contributor.alternativeName인동수-
dc.contributor.alternativeName유장렬-
dc.identifier.bibliographicCitationPlant Cell Tissue and Organ Culture, vol. 73, no. 1, pp. 15-19-
dc.identifier.doi10.1023/A:1022693605436-
dc.subject.keywordMyo-inositol-
dc.subject.keywordProtoplast culture-
dc.subject.keywordSomatic embryogenesis-
dc.subject.localMyo-inositol-
dc.subject.localProtoplast culture-
dc.subject.localprotoplast culture-
dc.subject.localsomatic embryogenisis-
dc.subject.localsomatic embryogenesis-
dc.subject.localSomatic embryogenesis-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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