DC Field | Value | Language |
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dc.contributor.author | Suk Weon Kim | - |
dc.contributor.author | Seung Chul Oh | - |
dc.contributor.author | D S In | - |
dc.contributor.author | Jang Ryol Liu | - |
dc.date.accessioned | 2017-04-19T09:00:07Z | - |
dc.date.available | 2017-04-19T09:00:07Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0167-6857 | - |
dc.identifier.uri | 10.1023/A:1022693605436 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6176 | - |
dc.description.abstract | Culture conditions are described for sustained cell division and plant regeneration from protoplasts of rose (Rosa hybrida L. 'Sumpath'). Protoplasts were enzymatically isolated from 2-week-old embryogenic cell suspension cultures. Freshly isolated protoplasts were plated as a thin layer onto protoplast culture medium (half-strength Murashige and Skoog's medium containing 60 g l-1 myo-inositol, 4.4 μM BA, and 1.4 μM 2,4-D) at a density of 5×104 protoplasts ml-1. The plating efficiency reached 3.9% after 2 weeks of culture. However, few protoplasts underwent cell division when cultured in protoplast culture medium in which 60 g l-1 myo-inositol was replaced with the same osmolarity of 90 g l-1 mannitol, indicating that myo-inositol is essential for sustained cell division of protoplasts. Colonies were formed after 8 weeks of culture at a frequency of 0.2%. Colonies were then transferred to colony culture medium (0.4% Gelrite-solidified protoplast culture medium) and maintained by subculturing at 4-week intervals to form embryogenic calluses. Upon transfer to half-strength MS basal medium, embryogenic calluses gave rise to numerous somatic embryos. Somatic embryos were transferred to half-strength MS basal medium containing 48 mg l-1 ferric ethylenediamine di-(o-hydroxyphenylacetate), where they subsequently developed into plantlets at a frequency of 30.9%. The plantlets had the same chromosome number of 2n=3x=21 as the source plant. They were successfully transplanted to potting soil and grown to maturity in a greenhouse. | - |
dc.publisher | Springer | - |
dc.title | Plant regeneration of rose (Rosa hybridia) from embryogenic cell-derived protoplasts | - |
dc.title.alternative | Plant regeneration of rose (Rosa hybridia) from embryogenic cell-derived protoplasts | - |
dc.type | Article | - |
dc.citation.title | Plant Cell Tissue and Organ Culture | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 19 | - |
dc.citation.startPage | 15 | - |
dc.citation.volume | 73 | - |
dc.contributor.affiliatedAuthor | Suk Weon Kim | - |
dc.contributor.affiliatedAuthor | Seung Chul Oh | - |
dc.contributor.affiliatedAuthor | Jang Ryol Liu | - |
dc.contributor.alternativeName | 김석원 | - |
dc.contributor.alternativeName | 오승철 | - |
dc.contributor.alternativeName | 인동수 | - |
dc.contributor.alternativeName | 유장렬 | - |
dc.identifier.bibliographicCitation | Plant Cell Tissue and Organ Culture, vol. 73, no. 1, pp. 15-19 | - |
dc.identifier.doi | 10.1023/A:1022693605436 | - |
dc.subject.keyword | Myo-inositol | - |
dc.subject.keyword | Protoplast culture | - |
dc.subject.keyword | Somatic embryogenesis | - |
dc.subject.local | Myo-inositol | - |
dc.subject.local | Protoplast culture | - |
dc.subject.local | protoplast culture | - |
dc.subject.local | somatic embryogenisis | - |
dc.subject.local | somatic embryogenesis | - |
dc.subject.local | Somatic embryogenesis | - |
dc.description.journalClass | Y | - |
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