Isolation of an angiotensin converting enzyme inhibitory substance from Chrysanthemum boreale makino

Cited 6 time in scopus
Metadata Downloads
Title
Isolation of an angiotensin converting enzyme inhibitory substance from Chrysanthemum boreale makino
Author(s)
J Kim; S H Lee; N Sun; Dong-Ho Choung; Won Gon KimSang Ku Lee; K B Song
Bibliographic Citation
Journal of Food Science, vol. 68, no. 3, pp. 816-819
Publication Year
2003
Abstract
Thirty plants of the Compositae family were screened for inhibitory activity of angiotensin converting enzyme (ACE) and Chrysanthemum boreale Makino was selected for further investigation since it had the highest inhibitory activity. Crude water extract of the flowers of Chrysanthemum boreale Makino was prepared by steeping in water at 95°C for 2 h, followed by centrifugation at 8000 × g for 30 min. Crude extract was then filtered using YM-3 and YM-1 membranes. An ACE inhibitor was isolated using consecutive chromatographic methods including: gel permeation chromatography, ion exchange chromatography, normal and reverse phase HPLC. The inhibitor was identified to be guanosine by mass spectrometry and NMR studies.
Keyword
ACE inhibitorChromatographyChrysanthemum boreale MakinoMass spectrometry
ISSN
0022-1147
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1365-2621.2003.tb08248.x
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.