α-Melanocyte-stimulating hormone inhibits lipopolysaccharide-induced tumor necrosis factor-α production in leukocytes by modulating protein kinase A, p38 kinase, and nuclear factor κB signaling pathway

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dc.contributor.authorSun Woo Yoon-
dc.contributor.authorSung-Ho Goh-
dc.contributor.authorJ S Chun-
dc.contributor.authorEun Wie Cho-
dc.contributor.authorMyung Kyu Lee-
dc.contributor.authorK L Kim-
dc.contributor.authorJ J Kim-
dc.contributor.authorC J Kim-
dc.contributor.authorHaryoung Poo-
dc.date.accessioned2017-04-19T09:00:12Z-
dc.date.available2017-04-19T09:00:12Z-
dc.date.issued2003-
dc.identifier.issn00219258-
dc.identifier.uri10.1074/jbc.M302444200ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6210-
dc.description.abstractThe neuropeptide α-melanocyte-stimulating hormone (α-MSH) inhibits inflammation by down-regulating the expression of proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) in leukocytes via stimulation of α-MSH cell surface receptors. However, the signaling mechanism of α-MSH action has not yet been clearly elucidated. Here, we have investigated signaling pathways by which α-MSH inhibits lipopolysaccharide (LPS)-induced TNF-α production in leukocytes such as THP-1 cells. We focused on the possible roles of protein kinase A (PKA), p38 kinase, and nuclear factor κB (NFκB) signaling. In THP-1 cells, LPS is known to activate p38 kinase, which in turn activates NFκB to induce TNF-α production. We found that pretreatment of cells with α-MSH blocked LPS-induced p38 kinase and NFκB activation as well as TNF-α production. This response was proportional to α-MSH receptor expression levels, and addition of an α-MSH receptor antagonist abolished the inhibitory effects. In addition, α-MSH treatment activated PKA, and PKA inhibition abrogated the inhibitory effects of α-MSH on p38 kinase activation, NFκB activation, and TNF-α production. Taken together, our results indicate that stimulation of PKA by α-MSH causes inhibition of LPS induced activation of p38 kinase and NFκB to block TNF-α production.-
dc.publisherAmer Soc Biochemistry Molecular Biology Inc-
dc.titleα-Melanocyte-stimulating hormone inhibits lipopolysaccharide-induced tumor necrosis factor-α production in leukocytes by modulating protein kinase A, p38 kinase, and nuclear factor κB signaling pathway-
dc.title.alternativeα-Melanocyte-stimulating hormone inhibits lipopolysaccharide-induced tumor necrosis factor-α production in leukocytes by modulating protein kinase A, p38 kinase, and nuclear factor κB signaling pathway-
dc.typeArticle-
dc.citation.titleJournal of Biological Chemistry-
dc.citation.number35-
dc.citation.endPage32920-
dc.citation.startPage32914-
dc.citation.volume278-
dc.contributor.affiliatedAuthorSun Woo Yoon-
dc.contributor.affiliatedAuthorEun Wie Cho-
dc.contributor.affiliatedAuthorMyung Kyu Lee-
dc.contributor.affiliatedAuthorHaryoung Poo-
dc.contributor.alternativeName윤선우-
dc.contributor.alternativeName고성호-
dc.contributor.alternativeName천장수-
dc.contributor.alternativeName조은위-
dc.contributor.alternativeName이명규-
dc.contributor.alternativeName김길룡-
dc.contributor.alternativeName김재진-
dc.contributor.alternativeName김철중-
dc.contributor.alternativeName부하령-
dc.identifier.bibliographicCitationJournal of Biological Chemistry, vol. 278, no. 35, pp. 32914-32920-
dc.identifier.doi10.1074/jbc.M302444200-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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