Molecular cloning and characterization of a Peroxiredoxin cDNA from cell cultures of sweetpotato = 고구마 배양세포에서 Peroxiredoxin cDNA의 분리 및 발현특성

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Title
Molecular cloning and characterization of a Peroxiredoxin cDNA from cell cultures of sweetpotato = 고구마 배양세포에서 Peroxiredoxin cDNA의 분리 및 발현특성
Author(s)
Soo Young Park; Sun Hwa Ryu; Suk Yoon Kwon; J G Kim; Sang Soo Kwak
Bibliographic Citation
Korean Journal of Plant Biotechnology (Tissue Culture), vol. 30, no. 2, pp. 135-141
Publication Year
2003
Abstract
Peroxiredoxin(Pix) are large family of peroxidases that reduce alkyl hydroperoxides and hydrogen peroxide. A cDNA clone (referred to as swPrxl) encoding Pix was from a sweetpotato cDNA library constructed from suspension-sultured cells, and its expression was investigated in terms of stress. The swPrxl contained an open reading frame (ORF) encoding mature protein of 193 amino acids with calculated molecular mass of 20.8kDa. The predicted amino acid sequence of swPrxl has two conserved cysteines that are essential resicues for the reduction of peroxides. It showed high amino acid sequence homology ot PixIIF of Arabidopsis (77%) and putative Prx of rice(72%). RNA gel-blot analysis showed that swPrxl gene was expressed dominantly in leave among intact tissues, and also highly detect in suspension-cultured cells. Interestingly, the level of swPrxl transcripts was almost the same regardless of the growth stage in suspension culture. Furthermore, the transcription level of swPrxl gene was not significantly changed in response to various stress treatments such as wounding, extreme temperature and stress-related chemicals RT-PCR analyses.
Keyword
cultured cellsenvironmental stressipomoea batatasperoxidaseperoxiredoxin
ISSN
1015-5880
Publisher
South Korea
DOI
http://dx.doi.org/10.5010/JPB.2003.30.2.135
Type
Article
Appears in Collections:
Division of Biomaterials Research > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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