DC Field | Value | Language |
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dc.contributor.author | Jae-Chan Lee | - |
dc.contributor.author | Hae Ryong Park | - |
dc.contributor.author | Dong Jin park | - |
dc.contributor.author | Y B Kim | - |
dc.contributor.author | Chang-Jin Kim | - |
dc.date.accessioned | 2017-04-19T09:00:14Z | - |
dc.date.available | 2017-04-19T09:00:14Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0257-2389 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6225 | - |
dc.description.abstract | Glycopeptide antibiotics, teicoplanin was purified from a mutant strain of Actinoplanes teichomyceticus ATCC31121, A. teichomyceticus MSL2211. We developed a simple procedure to separate and purify the teicoplanin from the fermentation broth. Teicoplanin was purified by two-step purification system, hydrophobic adsorption and sugar affinity chromatography in combination with HPLC analysis based on the properties of hydrophobic acyl chain and sugar moiety in teicoplanin. Teicoplanin was separated from the culture broth by Diaion HP-20 and further purified by concanavalin A affinity column chromatography. As an adsorbent resin, Diaion HP-20 in broth eliminated toxic effects on growth, reduced feedback repression of teicoplanin production, and assisted in rapid recovery of teicoplanin. The teicoplanin displayed the final yield of 80% and 95% of purity. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Separation and purification of teicoplanin by diaion HP-20 and concanavalin A chromatography = 소수성 diaion HP-20 및 친화성 concanavalin A 크로마토그래피를 이용한 glycopeptide계 항생제 teicoplanin의 분리 및 정제 | - |
dc.title.alternative | Separation and purification of teicoplanin by diaion HP-20 and concanavalin A chromatography | - |
dc.type | Article | - |
dc.citation.title | Korean Journal of (Applied) Microbiology & Biotechnology | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 204 | - |
dc.citation.startPage | 201 | - |
dc.citation.volume | 31 | - |
dc.contributor.affiliatedAuthor | Jae-Chan Lee | - |
dc.contributor.affiliatedAuthor | Hae Ryong Park | - |
dc.contributor.affiliatedAuthor | Dong Jin park | - |
dc.contributor.affiliatedAuthor | Chang-Jin Kim | - |
dc.contributor.alternativeName | 이재찬 | - |
dc.contributor.alternativeName | 박해룡 | - |
dc.contributor.alternativeName | 박동진 | - |
dc.contributor.alternativeName | 김영배 | - |
dc.contributor.alternativeName | 김창진 | - |
dc.identifier.bibliographicCitation | Korean Journal of (Applied) Microbiology & Biotechnology, vol. 31, no. 2, pp. 201-204 | - |
dc.subject.keyword | actinoplanes teicomyceticus | - |
dc.subject.keyword | concanavalin A | - |
dc.subject.keyword | diaion HP-20 | - |
dc.subject.keyword | teicoplanin | - |
dc.subject.local | Actinoplanes teicomyceticus | - |
dc.subject.local | actinoplanes teicomyceticus | - |
dc.subject.local | Concanavalin A. | - |
dc.subject.local | concanavalin A | - |
dc.subject.local | Concanavalin A | - |
dc.subject.local | diaion HP-20 | - |
dc.subject.local | Diaion HP-20 | - |
dc.subject.local | teicoplanin | - |
dc.subject.local | Teicoplanin | - |
dc.description.journalClass | N | - |
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