Gentiobiose synthesis from glucose using recombinant β-glucosidase from Thermus caldophilus GK24

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dc.contributor.authorT Y Kim-
dc.contributor.authorDae Sil Lee-
dc.contributor.authorH J Shin-
dc.date.accessioned2017-04-19T09:00:18Z-
dc.date.available2017-04-19T09:00:18Z-
dc.date.issued2003-
dc.identifier.issn1226-8372-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6244-
dc.description.abstractRecombinant β-glucosidase from Thermus caldophilus GK24 was easily purified partially by a heat treatment procedure, resulting in 8-fold and recovery yield of 80% from crude enzyme. When the β-glucosidase was incubated with a 80% glucose solution (w/w), gentiobiose (β1,6-glucobiose) was the major product in the reaction mixture. The optimal conditions for producing gentiobiose (11% yields of total sugar) were pH 8-9 and 70°C for 72 h.-
dc.publisherSpringer-
dc.titleGentiobiose synthesis from glucose using recombinant β-glucosidase from Thermus caldophilus GK24-
dc.title.alternativeGentiobiose synthesis from glucose using recombinant β-glucosidase from Thermus caldophilus GK24-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number0-
dc.citation.endPage212-
dc.citation.startPage210-
dc.citation.volume8-
dc.contributor.affiliatedAuthorDae Sil Lee-
dc.contributor.alternativeName김태연-
dc.contributor.alternativeName이대실-
dc.contributor.alternativeName신현재-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 8, pp. 210-212-
dc.identifier.doi10.1007/BF02935899-
dc.subject.keywordβ-glucosidase-
dc.subject.keywordgentiobiose-
dc.subject.keywordthermus caldophilus GK24-
dc.subject.localβ-Glucosidase-
dc.subject.localβ-glucosidase-
dc.subject.localΒ-glucosidase-
dc.subject.localgentiobiose-
dc.subject.localthermus caldophilus GK-24-
dc.subject.localthermus caldosphilus Gk24-
dc.subject.localThermus caldophilus GK-24-
dc.subject.localThermus caldophilus GK24-
dc.subject.localthermus caldophilus GK24-
dc.description.journalClassY-
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