Expression and localization of the transforming growth factor-β type I receptor and Smads in preneoplastic lesions during chemical hepatocarcinogenesis in rats

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Title
Expression and localization of the transforming growth factor-β type I receptor and Smads in preneoplastic lesions during chemical hepatocarcinogenesis in rats
Author(s)
D Y Park; C H Lee; M Y Sol; K S Suh; Sun Young Yoon; Jae Wha Kim
Bibliographic Citation
Journal of Korean Medical Science, vol. 18, no. 4, pp. 510-519
Publication Year
2003
Abstract
Little is known about the involvement of Smad-related molecules in the regulation of the Transforming Growth Factor (TGF)-β signaling pathway during hepatocarcinogenesis, particularly with respect to preneoplastic lesions of a rat liver. The aims of this study were to investigate the localizations and temporal expressions of TGF-β Receptor Type 1 (TGR1) and Smads during the promotion stage of chemical hepatocarcinogenesis in rats. We investigated expressions and localizations of TGR1, Smad2, Smad4, and Smad7 by using semi-quantitative RT-PCR and immunohistochemistry in preneoplastic lesions during rat chemical hepatocarcinogenesis induced by Solt and Farber's method. The down-regulation of TGR1, Smad2, and Smad4 was evident during the later steps of the promotion stage of chemical hepatocarcinogenesis. In contrast with other Smads, increased Smad7 expression was evident during the later steps of the promotion stage. Also immunohistochemistry revealed that the main site of TGR1, Smad2, Smad4, and Smad7 expression was mainly in hepatocytes of the preneoplastic lesions of a rat liver. Dysregulation of the downstream effectors of TGF-β such as TGR1, Smad2, Smad4 and, Smad7 might contribute to the progression of preneoplastic lesions during chemical hepatocarcinogenesis in a rat.
Keyword
CarcinogensLiverPrecancerous conditionsRatsReceptorsSignal transductionTransforming growth factor beta
ISSN
1011-8934
Publisher
South Korea
DOI
http://dx.doi.org/10.3346/jkms.2003.18.4.510
Type
Article
Appears in Collections:
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
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