Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags

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Title
Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tags
Author(s)
J D Jung; H W Park; Y Hahn; Cheol-Goo Hur; D S In; H J Chung; Jang Ryol Liu; D W Choi
Bibliographic Citation
Plant Cell Reports, vol. 22, no. 3, pp. 224-230
Publication Year
2003
Abstract
Expressed sequence tags (ESTs) provide a valuable tool that can be used to identify genes in secondary metabolite biosynthesis. Ginseng (Panax ginseng C.A Meyer) is a medicinal plant that accumulates ginsenosides in roots. We sequenced 11,636 ESTs from five ginseng libraries in order to create a gene resource for biosynthesis of ginsenosides, which are thought to be the major active component in roots. Only 59% of the ginseng ESTs exhibited significant homology to previously known polypeptide sequences. Stress- and pathogen-response proteins were most abundant in 4-year-old ginseng roots. ESTs involved in ginsenoside biosynthesis were identified by a keyword search of BLASTX results and a domain search of ginseng ESTs. We identified 4 oxidosqualene cyclase candidates involved in the cyclization reaction of 2,3-oxidosqualene, 9 nine cytochrome P450 and 12 glycosyltransferse candidates, which may be involved in modification of the triterpene backbone.
Keyword
Expressed sequence tagsGinsenosideOxidosqualene cyclasePanax ginsengSecondary metabolism
ISSN
0721-7714
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00299-003-0678-6
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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