DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yeo Jin Jung | - |
dc.contributor.author | Jung-Kee Lee | - |
dc.contributor.author | C G Sung | - |
dc.contributor.author | Tae Kwang Oh | - |
dc.contributor.author | Hyung Kwoun Kim | - |
dc.date.accessioned | 2017-04-19T09:00:24Z | - |
dc.date.available | 2017-04-19T09:00:24Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1381-1177 | - |
dc.identifier.uri | 10.1016/j.molcatb.2003.06.006 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6275 | - |
dc.description.abstract | An esterase-producing Bacillus megaterium strain (20-1) was isolated from a soil sample collected in South Korea. The cloned gene showed that the esterase 20-1 composed of 310 amino acids corresponding to a molecular mass (Mr) of 34,638. Based on the Mr and the protein sequence, the esterase 20-1 belonged to the H lipase/esterase group. The optimum temperature and pH of the purified His-tagged enzyme were 20-35°C and 8.0, respectively. The esterase 20-1 showed a 'nonionic detergent-induced activation' phenomenon, which was a detergent type- and concentration-dependent process. In comparison with the native enzyme, the Tween 80-treated enzyme had relatively a similar kcat value of 274s-1 but a very low Km value of 0.037mM for PNPC (C6), therefore, it showed a 14-fold increase in kcat/Km value. | - |
dc.publisher | Elsevier | - |
dc.title | Nonionic detergent-induced activation of an esterase from Bacillus megaterium 20-1 | - |
dc.title.alternative | Nonionic detergent-induced activation of an esterase from Bacillus megaterium 20-1 | - |
dc.type | Article | - |
dc.citation.title | Journal of Molecular Catalysis B: Enzymatic | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 229 | - |
dc.citation.startPage | 223 | - |
dc.citation.volume | 26 | - |
dc.contributor.affiliatedAuthor | Yeo Jin Jung | - |
dc.contributor.affiliatedAuthor | Jung-Kee Lee | - |
dc.contributor.affiliatedAuthor | Tae Kwang Oh | - |
dc.contributor.alternativeName | 정여진 | - |
dc.contributor.alternativeName | 이정기 | - |
dc.contributor.alternativeName | 성창근 | - |
dc.contributor.alternativeName | 오태광 | - |
dc.contributor.alternativeName | 김형권 | - |
dc.identifier.bibliographicCitation | Journal of Molecular Catalysis B: Enzymatic, vol. 26, no. 3, pp. 223-229 | - |
dc.identifier.doi | 10.1016/j.molcatb.2003.06.006 | - |
dc.subject.keyword | Alicyclobacillus acidocaldarius | - |
dc.subject.keyword | Bacillus megaterium | - |
dc.subject.keyword | Detergent-induced activation | - |
dc.subject.keyword | Esterase | - |
dc.subject.local | Alicyclobacillus acidocaldarius | - |
dc.subject.local | Bacillus megaterium | - |
dc.subject.local | Detergent-induced activation | - |
dc.subject.local | Esterase | - |
dc.subject.local | esterases | - |
dc.subject.local | esterase | - |
dc.description.journalClass | N | - |
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