Elevation of serum asialo-α1 acid glycoprotein concentration in patients with hepatic cirrhosis and hepatocellular carcinoma as measured by antibody-lectin sandwich assay

Cited 22 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorEun Young Song-
dc.contributor.authorK A Kim-
dc.contributor.authorY D Kim-
dc.contributor.authorE Y Lee-
dc.contributor.authorHong Soo Lee-
dc.contributor.authorH J Kim-
dc.contributor.authorB M Ahn-
dc.contributor.authorYong Kyung Choe-
dc.contributor.authorC H Kim-
dc.contributor.authorT W Chung-
dc.date.accessioned2017-04-19T09:00:25Z-
dc.date.available2017-04-19T09:00:25Z-
dc.date.issued2003-
dc.identifier.issn1386-6346-
dc.identifier.uri10.1016/S1386-6346(03)00156-6ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6280-
dc.description.abstractSerum asialoglycoproteins (desialylated glycoproteins) concentration was reported to be elevated in patients with hepatic disease as compared with that of normal subjects. In this study, we measured serum asialo-α1 acid glycoprotein (AsAGP) level by a solid-phase sandwich assay in which monoclonal antibody (mAb) to α1-acid glycoprotein and galactose-binding lectin, ricinus communis (RCA), have been employed as capture protein and probe protein, respectively. The mAb-RCA sandwich assay was sensitive (0.02 μg/ml) and specific for AsAGP. We have determined AsAGP concentration of 869 serum specimens and analyzed the results using 1.38 and 2.24 μg/ml (AsAGP) as cut-off values, respectively. AsAGP level was 0.80 ± 0.29 μg/ml (mean ± S.D.) with 97 normal serum specimens and elevated primarily in patients with liver cirrhosis (LC) or hepatocellular carcinoma (HCC). Using 1.38 μg/ml as a cutoff, 4/97 normal subjects, 11/39 acute hepatitis and 26/159 non-hepatic disease exhibited a slight elevation, whereas, AsAGP level was significantly elevated in 182/230 LC and 63/72 HCC. Meanwhile, a cutoff of 2.24 μg/ml allowed significant differentiation of LC or HCC from chronic hepatitis. Serum AsAGP level appeared to increase progressively with increasing severity of liver disease in cirrhotic patients. Thus, serum AsAGP concentration, as measured by the new mAb-RCA sandwich assay, may be a useful differential marker as a diagnostic aid for LC or HCC.-
dc.publisherWiley-
dc.titleElevation of serum asialo-α1 acid glycoprotein concentration in patients with hepatic cirrhosis and hepatocellular carcinoma as measured by antibody-lectin sandwich assay-
dc.title.alternativeElevation of serum asialo-α1 acid glycoprotein concentration in patients with hepatic cirrhosis and hepatocellular carcinoma as measured by antibody-lectin sandwich assay-
dc.typeArticle-
dc.citation.titleHepatology Research-
dc.citation.number4-
dc.citation.endPage317-
dc.citation.startPage311-
dc.citation.volume26-
dc.contributor.affiliatedAuthorEun Young Song-
dc.contributor.affiliatedAuthorHong Soo Lee-
dc.contributor.affiliatedAuthorYong Kyung Choe-
dc.contributor.alternativeName송은영-
dc.contributor.alternativeName김경아-
dc.contributor.alternativeName김영대-
dc.contributor.alternativeName이은영-
dc.contributor.alternativeName이홍수-
dc.contributor.alternativeName김희정-
dc.contributor.alternativeName안병민-
dc.contributor.alternativeName최용경-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName정태화-
dc.identifier.bibliographicCitationHepatology Research, vol. 26, no. 4, pp. 311-317-
dc.identifier.doi10.1016/S1386-6346(03)00156-6-
dc.subject.keywordAntibody-lectin sandwich assay-
dc.subject.keywordAsialo-α1 acid glycoprotein-
dc.subject.keywordAsialoglycoproteins-
dc.subject.keywordHepatic disease serum marker-
dc.subject.localAntibody-lectin sandwich assay-
dc.subject.localAsialo-α1-acid glycoprotein-
dc.subject.localAsialo-α1 acid glycoprotein-
dc.subject.localAsialoglycoproteins-
dc.subject.localHepatic disease serum marker-
dc.description.journalClassY-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.