Expression of human β-amyloid peptide in transgenic potato

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dc.contributor.authorHyun Soon Kim-
dc.contributor.authorJoung Won Euym-
dc.contributor.authorMi Sun Kim-
dc.contributor.authorByung Chan Lee-
dc.contributor.authorI Mook-Jung-
dc.contributor.authorJae Heung Jeon-
dc.contributor.authorHyouk Joung-
dc.date.accessioned2017-04-19T09:00:26Z-
dc.date.available2017-04-19T09:00:26Z-
dc.date.issued2003-
dc.identifier.issn0168-9452-
dc.identifier.uri10.1016/j.plantsci.2003.07.007ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6286-
dc.description.abstractThe insoluble β-amyloid peptide is associated with the development of Alzheimer's disease, β-amyloid cDNA was cloned from human wild-type APP695 cDNA. Transgenic potato (Solanum tuberosum L.) plants were then developed by introducing the gene encoding β-amyloid, under the control of CaMV 35S promoter. The presence of the ß-amyloid gene in the plant genome was verified by PCR and Southern hybridization experiments. Northern blot analysis showed that the genes coding for the β-amyloid antigen could be expressed in potato plants. Three lines of transgenic plant that express high levels of mRNA were screened in a further analysis. The expression of recombinant β-amyloid was then observed in transgenic plants by ELISA using the specific antibody developed, the results showed three- to fivefold higher than non-transgenic potatoes. The morphological characteristics, including shoot growth and tuberization were investigated between non-transgenic and transgenic potato plants. No significant differences in shoot growth and tuberization were observed between the non-transgenic and transgenic plants.-
dc.publisherElsevier-
dc.titleExpression of human β-amyloid peptide in transgenic potato-
dc.title.alternativeExpression of human β-amyloid peptide in transgenic potato-
dc.typeArticle-
dc.citation.titlePlant Science-
dc.citation.number6-
dc.citation.endPage1451-
dc.citation.startPage1445-
dc.citation.volume165-
dc.contributor.affiliatedAuthorHyun Soon Kim-
dc.contributor.affiliatedAuthorJoung Won Euym-
dc.contributor.affiliatedAuthorMi Sun Kim-
dc.contributor.affiliatedAuthorByung Chan Lee-
dc.contributor.affiliatedAuthorJae Heung Jeon-
dc.contributor.affiliatedAuthorHyouk Joung-
dc.contributor.alternativeName김현순-
dc.contributor.alternativeName염정원-
dc.contributor.alternativeName김미선-
dc.contributor.alternativeName이병찬-
dc.contributor.alternativeName묵정인희-
dc.contributor.alternativeName전재흥-
dc.contributor.alternativeName정혁-
dc.identifier.bibliographicCitationPlant Science, vol. 165, no. 6, pp. 1445-1451-
dc.identifier.doi10.1016/j.plantsci.2003.07.007-
dc.subject.keywordAgrobacterium-mediated transformation-
dc.subject.keywordAlzheimer's disease-
dc.subject.keywordMicrotuberization-
dc.subject.keywordSolanum tuberosum L.-
dc.subject.localAgrobacterium-mediated transformation-
dc.subject.localagrobacterium-mediated transformation-
dc.subject.localalzheimer's disease-
dc.subject.localAlzheimer’s disease (AD)-
dc.subject.localAlzheimer’s disease-
dc.subject.localAlzheimer's Disease-
dc.subject.localAlzheimer disease-
dc.subject.localAlzheimer's disease (AD)-
dc.subject.localAlzheimer′s disease-
dc.subject.localAlzheimer's disease-
dc.subject.localMicrotuberization-
dc.subject.localSolanum tuberosum L.-
dc.subject.localSolanum tuberosum-
dc.subject.localsolanum tuberosum-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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