Suppression of RelA/p65 transactivation activity by a lignoid manassantin isolated from Saururus chinensis

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dc.contributor.authorJeong-Hyung Lee-
dc.contributor.authorB Y Hwang-
dc.contributor.authorKyung Sook Kim-
dc.contributor.authorJeong Beom Nam-
dc.contributor.authorYoung-Soo Hong-
dc.contributor.authorJung Joon Lee-
dc.date.accessioned2017-04-19T09:00:28Z-
dc.date.available2017-04-19T09:00:28Z-
dc.date.issued2003-
dc.identifier.issn00062952-
dc.identifier.uri10.1016/S0006-2952(03)00553-7ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6299-
dc.description.abstractIn our search for NF-κB inhibitors from natural resources, we have previously identified two structurally related dilignans, manassantin A and B as specific inhibitors of NF-κB activation from Saururus chinensis. However, their molecular mechanism of action remains unclear. We here demonstrate that manassantins A and B are potent inhibitors of NF-κB activation by the suppression of transciptional activity of RelA/p65 subunit of NF-κB. These compounds significantly inhibited the induced expression of NF-κB reporter gene by LPS or TNF-α in a dose-dependent manner. However, these compounds did not prevent the DNA-binding activity of NF-κB assessed by electrophoretic mobility shift assay as well as the induced-degradation of IκB-α protein by LPS or TNF-α. Further analysis revealed that manassantins A and B dose-dependently suppressed not only the induced NF-κB activation by overexpression of RelA/p65, but also transactivation activity of RelA/p65. Furthermore, treatment of cells with these compounds prevented the TNF-α-induced expression of anti-apoptotic NF-κB target genes Bfl-1/A1, a prosurvival Bcl-2 homologue, and resulted in sensitizing HT-1080 cells to TNF-α-induced cell death. Similarly, these compounds also suppressed the LPS-induced inducible nitric oxide synthase expression and nitric oxide production. Taken together, manassantins A and B could be valuable candidate for the intervention of NF-κB-dependent pathological condition such as inflammation and cancer.-
dc.publisherElsevier-
dc.titleSuppression of RelA/p65 transactivation activity by a lignoid manassantin isolated from Saururus chinensis-
dc.title.alternativeSuppression of RelA/p65 transactivation activity by a lignoid manassantin isolated from Saururus chinensis-
dc.typeArticle-
dc.citation.titleBiochemical Pharmacology-
dc.citation.number10-
dc.citation.endPage1933-
dc.citation.startPage1925-
dc.citation.volume66-
dc.contributor.affiliatedAuthorYoung-Soo Hong-
dc.contributor.alternativeName이정형-
dc.contributor.alternativeName황방연-
dc.contributor.alternativeName김경숙-
dc.contributor.alternativeName남정범-
dc.contributor.alternativeName홍영수-
dc.contributor.alternativeName이정준-
dc.identifier.bibliographicCitationBiochemical Pharmacology, vol. 66, no. 10, pp. 1925-1933-
dc.identifier.doi10.1016/S0006-2952(03)00553-7-
dc.subject.keywordapoptosis-
dc.subject.keywordlignoids-
dc.subject.keywordNF-κB-
dc.subject.keywordRelA/p65-
dc.subject.keywordsaururus chinensis-
dc.subject.keywordtransactivation-
dc.subject.localapoptosis-
dc.subject.localApoptosis-
dc.subject.locallignoids-
dc.subject.localNF-κB-
dc.subject.localNF-kB-
dc.subject.localRelA/p65-
dc.subject.localsaururus chinensis-
dc.subject.localSaururus chinensis-
dc.subject.localtransactivation-
dc.subject.localTransactivation-
dc.description.journalClassY-
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Ochang Branch Institute > Anticancer Agent Research Center > 1. Journal Articles
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