Properties of the Hansenula polymorpha-derived constitutive GAP promoter, assessed using an HSA reporter gene

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dc.contributor.authorJoo Hyung Heo-
dc.contributor.authorWon Kyoung Hong-
dc.contributor.authorE Y Cho-
dc.contributor.authorMoo Woong Kim-
dc.contributor.authorJ Y Kim-
dc.contributor.authorChul Ho Kim-
dc.contributor.authorSang Ki Rhee-
dc.contributor.authorHyun Ah Kang-
dc.description.abstractThe glyceraldehyde-3-phosphate dehydrogenase promoter, PGAP, was employed to direct the constitutive expression of recombinant human serum albumin (HSA) in Hansenula polymorpha. A set of integration vectors containing the HSA cDNA under the control of PGAP was constructed and the elemental parameters affecting the expression of HSA from PGAP were analyzed. The presence of a 5′-untranslated region derived from the HSA cDNA and the integration of the expression vector into the GAP locus were shown to improve the expression of HSA under PGAP. Glycerol supported a higher level of HSA expression from PGAP along with a higher cell density than either glucose or methanol. The growth at high glycerol concentrations up to 12% did not cause any significant repression of the cell growth. A high cell density culture, up to 83 g l-1 dry cell weight with a HSA production of 550 mg l-1, was obtained in less than 32 h of cultivation in a fed-batch fermentation employing intermittent feeding with 12% glycerol. The GAP promoter-based HSA expression system showed a higher specific production rate and required a much simpler fermentation process than the MOX promoter-based system, demonstrating that PGAP can be a practical alternative of the MOX promoter in the large-scale production of HSA from H. polymorpha.-
dc.publisherOxford Univ Press-
dc.titleProperties of the Hansenula polymorpha-derived constitutive GAP promoter, assessed using an HSA reporter gene-
dc.title.alternativeProperties of the Hansenula polymorpha-derived constitutive GAP promoter, assessed using an HSA reporter gene-
dc.citation.titleFEMS Yeast Research-
dc.contributor.affiliatedAuthorJoo Hyung Heo-
dc.contributor.affiliatedAuthorWon Kyoung Hong-
dc.contributor.affiliatedAuthorMoo Woong Kim-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.affiliatedAuthorHyun Ah Kang-
dc.identifier.bibliographicCitationFEMS Yeast Research, vol. 4, no. 2, pp. 175-184-
dc.subject.keywordconstitutive expression-
dc.subject.keywordglyceraldehyde-3-phosphate dehydrogenase promoter-
dc.subject.keywordhansenula polymorpha-
dc.subject.keywordhigh cell density culture-
dc.subject.keywordhuman serum albumin-
dc.subject.localconstitutive expression-
dc.subject.localConstitutive expression-
dc.subject.localglyceraldehyde-3-phosphate dehydrogenase promoter-
dc.subject.localhansenula polymorpha-
dc.subject.localHansenula polymorpha-
dc.subject.localHansenula polymorpha (Pichia angusta)-
dc.subject.localhigh cell density culture-
dc.subject.localHuman serum albumin-
dc.subject.localhuman serum albumin-
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Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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