Stability of murine, chimeric and humanized antibodies against pre-S2 surface antigen of hepatitis B virus

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Title
Stability of murine, chimeric and humanized antibodies against pre-S2 surface antigen of hepatitis B virus
Author(s)
Sung Sup Park; J H Kim; J F Brandts; Hyo Jeong Hong
Bibliographic Citation
Biologicals, vol. 31, no. 4, pp. 295-302
Publication Year
2003
Abstract
We have constructed a humanized antibody with specificity for the pre-S2 surface antigen of hepatitis B virus (HBV) by grafting the complementarity determining regions (CDRs) of parental murine monoclonal antibody (mAb) into human anti-Sm antibody framework regions. The humanized antibody has a substitution at position 94 in a framework region of the heavy chain variable region, and exhibits the same antigen binding affinity as the parental murine monoclonal and chimeric antibodies. In order to assess the stability of these antibodies, thermal inactivation of the parental, chimeric and humanized antibodies was analyzed. Fifty percent inactivation of the chimeric and humanized antibodies was observed at 63.7°C and 68.7°C, respectively, compared to 55.0°C for murine antibody. The humanized antibody also exhibited increased stability against denaturant. Guanidine-induced unfolding monitored by the changes in fluorescence intensity at 360 nm showed that midpoints of the transition of the chimeric and humanized antibodies were 2.47 M and 2.56 M, respectively, whereas that of the murine antibody was 1.36 M.
Keyword
humanized antibodymicrocalorimetryPre-S2stability
ISSN
1045-1056
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biologicals.2003.08.003
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
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