DC Field | Value | Language |
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dc.contributor.author | A G Wang | - |
dc.contributor.author | Sun-Uk Kim | - |
dc.contributor.author | Y H Han | - |
dc.contributor.author | S K Kim | - |
dc.contributor.author | Dae Yeul Yu | - |
dc.date.accessioned | 2017-04-19T09:00:38Z | - |
dc.date.available | 2017-04-19T09:00:38Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | I000-0006 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/6343 | - |
dc.description.abstract | The objective of this study is to improve the efficiency of embryo transfer in generation of transgenic mice using inbred mouse (C57BL/6J) embryos. The embryos of C57BL/6J and BCF1 mice were superovulated by the standard protocol. One-cell stage of embryos were microinjected and the resulted one-or two-cell were transferred into one-or two-side oviducts of foster mother, respectively. When one-cell stage of embryos were transferred into one-side oviduct of 0.75 d.p.c. foster mother, the results were not ideal because of showing pregnancy ratios of 68.8±7.83% for C57BL/6J and 48.3±14.22% for BCF1, and development ratios of pups vs transferred embryos of 11.9±5.51% for C57BL/6J and 10.5±8.03% fur BCF1 . However, when two-cell stage of embryos were transferred into two-side oviducts of 0.5 d.p.c. foster mother, we got better results of 94.4±9.64% and 100±0% pregnancy ratio, and 22.1±0.4% and 21.8±0.38% development ratio for C57BL/6J and BCF1, respectively. Therefore, transferring two-cell stage of C57BL/6J embryos into two-side oviducts of 0.5 d.p.c. foster mother may be competitive to the result in BCF1 embryos. Even if there are a lot of other factors affecting these results, we conclude from these experiments that transfer of two-cell embryos into two-side oviducts of 0.5 d.p.c. foster mother is a more efficient and safe method than others in generating transgenic mice using inbred mice embryos. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Studies on the efficient embryo transfer methods using inbred embryos in generation of transgenic mice = Inbred 마우스 수정란을 이용한 형질전환마우스 생산에 있어서의 효과적인 수정란 이식 방법에 관한 연구 | - |
dc.title.alternative | Studies on the efficient embryo transfer methods using inbred embryos in generation of transgenic mice | - |
dc.type | Article | - |
dc.citation.title | Reproductive & Developmental Biology | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 75 | - |
dc.citation.startPage | 69 | - |
dc.citation.volume | 27 | - |
dc.contributor.affiliatedAuthor | A G Wang | - |
dc.contributor.affiliatedAuthor | Sun-Uk Kim | - |
dc.contributor.affiliatedAuthor | Y H Han | - |
dc.contributor.affiliatedAuthor | Dae Yeul Yu | - |
dc.contributor.alternativeName | 왕애국 | - |
dc.contributor.alternativeName | 김선욱 | - |
dc.contributor.alternativeName | 한영호 | - |
dc.contributor.alternativeName | 김상근 | - |
dc.contributor.alternativeName | 유대열 | - |
dc.identifier.bibliographicCitation | Reproductive & Developmental Biology, vol. 27, no. 1, pp. 69-75 | - |
dc.subject.keyword | inbred mouse | - |
dc.subject.keyword | embryo transfer | - |
dc.subject.keyword | superovulation | - |
dc.subject.local | inbred mouse | - |
dc.subject.local | Embryo transfer | - |
dc.subject.local | embryo transfer | - |
dc.subject.local | Superovulation | - |
dc.subject.local | superovulation | - |
dc.description.journalClass | N | - |
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