Studies on the efficient embryo transfer methods using inbred embryos in generation of transgenic mice = Inbred 마우스 수정란을 이용한 형질전환마우스 생산에 있어서의 효과적인 수정란 이식 방법에 관한 연구

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorA G Wang-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorY H Han-
dc.contributor.authorS K Kim-
dc.contributor.authorDae Yeul Yu-
dc.date.accessioned2017-04-19T09:00:38Z-
dc.date.available2017-04-19T09:00:38Z-
dc.date.issued2003-
dc.identifier.issnI000-0006-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6343-
dc.description.abstractThe objective of this study is to improve the efficiency of embryo transfer in generation of transgenic mice using inbred mouse (C57BL/6J) embryos. The embryos of C57BL/6J and BCF1 mice were superovulated by the standard protocol. One-cell stage of embryos were microinjected and the resulted one-or two-cell were transferred into one-or two-side oviducts of foster mother, respectively. When one-cell stage of embryos were transferred into one-side oviduct of 0.75 d.p.c. foster mother, the results were not ideal because of showing pregnancy ratios of 68.8±7.83% for C57BL/6J and 48.3±14.22% for BCF1, and development ratios of pups vs transferred embryos of 11.9±5.51% for C57BL/6J and 10.5±8.03% fur BCF1 . However, when two-cell stage of embryos were transferred into two-side oviducts of 0.5 d.p.c. foster mother, we got better results of 94.4±9.64% and 100±0% pregnancy ratio, and 22.1±0.4% and 21.8±0.38% development ratio for C57BL/6J and BCF1, respectively. Therefore, transferring two-cell stage of C57BL/6J embryos into two-side oviducts of 0.5 d.p.c. foster mother may be competitive to the result in BCF1 embryos. Even if there are a lot of other factors affecting these results, we conclude from these experiments that transfer of two-cell embryos into two-side oviducts of 0.5 d.p.c. foster mother is a more efficient and safe method than others in generating transgenic mice using inbred mice embryos.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleStudies on the efficient embryo transfer methods using inbred embryos in generation of transgenic mice = Inbred 마우스 수정란을 이용한 형질전환마우스 생산에 있어서의 효과적인 수정란 이식 방법에 관한 연구-
dc.title.alternativeStudies on the efficient embryo transfer methods using inbred embryos in generation of transgenic mice-
dc.typeArticle-
dc.citation.titleReproductive & Developmental Biology-
dc.citation.number1-
dc.citation.endPage75-
dc.citation.startPage69-
dc.citation.volume27-
dc.contributor.affiliatedAuthorA G Wang-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorY H Han-
dc.contributor.affiliatedAuthorDae Yeul Yu-
dc.contributor.alternativeName왕애국-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName한영호-
dc.contributor.alternativeName김상근-
dc.contributor.alternativeName유대열-
dc.identifier.bibliographicCitationReproductive & Developmental Biology, vol. 27, no. 1, pp. 69-75-
dc.subject.keywordinbred mouse-
dc.subject.keywordembryo transfer-
dc.subject.keywordsuperovulation-
dc.subject.localinbred mouse-
dc.subject.localEmbryo transfer-
dc.subject.localembryo transfer-
dc.subject.localSuperovulation-
dc.subject.localsuperovulation-
dc.description.journalClassN-
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.