Independent exponential feeding of glycerol and methanol for fed-batch culture of recombinant Hansenula polymorpha DL-1

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Title
Independent exponential feeding of glycerol and methanol for fed-batch culture of recombinant Hansenula polymorpha DL-1
Author(s)
H Moon; S W Kim; J Lee; Sang Ki Rhee; Eui Sung Choi; Hyun Ah Kang; I H Kim; S I Hong
Bibliographic Citation
Applied Biochemistry and Biotechnology, vol. 111, no. 2, pp. 65-80
Publication Year
2003
Abstract
As a novel feeding strategy for optimizing human epidermal growth factor (hEGF) production with a recombinant Hansenula polymorpha DL-1 using the methanol oxidase (MOX) promoter in H. polymorpha DL-1, independent exponential feeding of two substrates was used. A simple kinetic model considering the cell growth on two substrates was established and used to calculate the respective feeding rates of glycerol and methanol. In the fed-batch culture with methanol-only feeding, the optimal set point of specific growth rate on methanol was found to be 0.10 h-1. When the fed-batch cultures were conducted by the independent feeding of glycerol and methanol, the actual specific growth rate on glycerol and methanol was slightly lower than the set point of specific growth rate. By the uncoupled feeding of glycerol and methanol the volumetric productivity of hEGF increased from 6.4 to 8.0 mg/(L·h), compared with methanol-only feeding.
Keyword
Exponential feedingFed-batch cultureHansenula polymorphaHuman epidermal growth factorMixed feed
ISSN
0273-2289
Publisher
Springer
DOI
http://dx.doi.org/10.1385/ABAB:111:2:65
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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