Application of denaturing gradient gel electrophoresis to estimate the diversity of commensal thermophiles

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Title
Application of denaturing gradient gel electrophoresis to estimate the diversity of commensal thermophiles
Author(s)
Jin-Woo Bae; J J Kim; Che Ok Jeon; K Kim; Jae Jun SongSeung Goo LeeHaryoung Poo; C M Jung; Yong Ha Park; M H Sung
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 13, no. 6, pp. 1008-1012
Publication Year
2003
Abstract
Symbiobacterium toebii has been reported as a thermophile exhibiting a commensal interaction with Geobacillus toebii. The distribution of the commensal thermophiles in various soils was investigated using a denaturing gradient gel electrophoresis (DGGE). Based on the DGGE analysis, the enrichment condition for the growth of Symbiobacterium sp. was found to also enrich populations of several other microbial spp. as well as Symbiobacterium sp. In the enrichment experiment, several different 16S rDNA sequences of commensal thermophiles were detected in all of the soil samples tested, indicating that commensal thermophiles are widely distributed in various soils.
Keyword
DGGEdiversitysymbiobacterium toebii
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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