Purification and characterization of phospholipase D from actinomycetes KF923 = 방선균 KF923이 생산하는 phospholipase D의 정제 및 특성

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dc.contributor.authorB Y Kwak-
dc.contributor.authorS H Yoon-
dc.contributor.authorChang-Jin Kim-
dc.contributor.authorD H Shon-
dc.date.accessioned2017-04-19T09:00:42Z-
dc.date.available2017-04-19T09:00:42Z-
dc.date.issued2003-
dc.identifier.issn0257-2389-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6368-
dc.description.abstractIn order to screen microorganisms producing phopholipase D (PLD) had high transphosphatidylation activity, about 1,000 Actinomycetes strains were isolated from the 63 soil samples, collected over 6 local area in Korea. When the hydrolytic activity in the supernatant was determined, 131 strains produced PLD more than 0.3 U/ml. Among 131 culture broths tested, 23 ones had transphosphatidylation activity higher than 20% and finally one strain (Actinomycetes KF 923), which had highest hydrolytic and transphophadylation activity, was selected. Actinomycetes KF923 showed the highest hydrolytic activity (13 U/ml) and phosphatidylation activity (95%) after 48 h fermentation using the P medium (yeast extract 1%, peptone 1%, glucose 1.5%, glycerol 1%, CaCO3 0.4%, pH 7.2). PLD was purified from the culture broth of Actinomycetes KF923 and the specific activity of purified PLD was 567 U/mg. The molecular weight of PLD was about 55 kD and the optimum pH and temperature were 6.0 and 60°C, respectively. The stability of PLD toward pH and temperature were high around pH 8.0 and below 40°C. Special metal ions were not necessary to the PLD activity.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titlePurification and characterization of phospholipase D from actinomycetes KF923 = 방선균 KF923이 생산하는 phospholipase D의 정제 및 특성-
dc.title.alternativePurification and characterization of phospholipase D from actinomycetes KF923-
dc.typeArticle-
dc.citation.titleKorean Journal of (Applied) Microbiology & Biotechnology-
dc.citation.number4-
dc.citation.endPage394-
dc.citation.startPage389-
dc.citation.volume31-
dc.contributor.affiliatedAuthorChang-Jin Kim-
dc.contributor.alternativeName곽보연-
dc.contributor.alternativeName윤석후-
dc.contributor.alternativeName김창진-
dc.contributor.alternativeName손동화-
dc.identifier.bibliographicCitationKorean Journal of (Applied) Microbiology & Biotechnology, vol. 31, no. 4, pp. 389-394-
dc.subject.keywordactinomycetes-
dc.subject.keywordcharacteristics-
dc.subject.keywordphospholipase D-
dc.subject.keywordpurification-
dc.subject.localactinomycete-
dc.subject.localActinomycetes-
dc.subject.localactinomycetes-
dc.subject.localcharacteristics-
dc.subject.localCharacteristics-
dc.subject.localphospholipase D-
dc.subject.localPhospholipase D-
dc.subject.localPurification-
dc.subject.localpurification-
dc.subject.localPurifcation-
dc.description.journalClassN-
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