Effects of oil contamination levels and microbial size on hydrocarbon biodegradation = 원유오염농도와 미생물 농도가 탄화수소의 생분해에 미치는 영향

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Title
Effects of oil contamination levels and microbial size on hydrocarbon biodegradation = 원유오염농도와 미생물 농도가 탄화수소의 생분해에 미치는 영향
Author(s)
Kyung Hwa Baek; Hee Sik Kim; I S Lee; Hee-Mock Oh; Byung Dae Yoon
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 31, no. 4, pp. 408-412
Publication Year
2003
Abstract
The purpose of this study was to evaluate the influence of oil concentration and inoculum size on petroleum biodegradation in soil by Nocardia sp. H17-1, isolated from oil-contaminated soil. To investigate the effect of initial oil concentration on total petroleum hydrocarbon (TPH) degradation, the soil was artificially contaminated with 10, 50 or 100 g of Arabian light oil per kg of soil, respectively. After 50 days, Nocardia sp. H17-1 degraded 78, 94 and 53% of the each initial TPH concentration, respectively. Also, it produced 1.35, 4.21, and 5.91 mmol of CO2 per g of soil, respectively. The degradation rate constant (k) of TPH was decreased in proportion to the initial oil concentrations while CO2 production was increased with the concentration. The growth of Nocardia sp. H17-1 was remarkably inhibited when it was inoculated into soil containing 100 g of oil per kg of soil. To evaluate the effect of the inoculum size, the soil was artificially contaminated with 50 g of Arabian light oil per kg of soil, and inoculated with 3×10 6, 5×107, 2×108 cells per g of soil, respectively. After 50 days, the degradation of TPH was remained with similar in all treatment but degradation rate constant (k) and evolved CO 2 was increased with increasing the inoculum size.
Keyword
biodegradationinoculum sizenocardia sp. H17-1oil concentrationtotal petroleum hydrocarbon (TPH)
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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