Differentiation of actinomycete genera based on partial rpoB gene sequences = rpoB 유전자 부분 분석에 의한 방선균 속 동정

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Title
Differentiation of actinomycete genera based on partial rpoB gene sequences = rpoB 유전자 부분 분석에 의한 방선균 속 동정
Author(s)
B J Kim; Y H Koh; J S Chun; Chang-Jin Kim; S H Lee; M J Cho; J W Hyun; K H Lee; C Y Cha; Y H Kook
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 13, no. 6, pp. 846-852
Publication Year
2003
Abstract
rpoB DNAs (279 bp) from 34 species of 5 actinomycete genera were sequenced and a phylogenetic tree was constructed based on the sequences obtained. The genera were clearly differentiated in the rpoB tree, forming clades specific to their respective genus. In addition, 2 signature amino acid residues specific to Streptomyces were found in a multiple alignment of the deduced amino acid sequences. To empirically confirm that this rpoB gene analysis system could be used to differentiate actinomycete isolates, the proposed system was used to identify 16 actinomycete isolates from Jeju Island. All isolates were successfully differentiated into the genera Streptomyces and Micromonospora. Accordingly, this is the first report that an rpoB sequence analysis has been effectively used to differentiate actinomycete strains at the genus level.
Keyword
actinomycete generadifferentiationidentificationphylogenetic relationshiprpoBsignature amino acids
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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