Isolation and characterization of a Lymantria dispar multinucleocapsid nucleopolyhedrovirus isolate in Korea

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Title
Isolation and characterization of a Lymantria dispar multinucleocapsid nucleopolyhedrovirus isolate in Korea
Author(s)
H J Shim; J Y Roh; J Y Choi; M S Li; S D Woo; Hyun Woo Oh; K S Boo; Y H Je
Bibliographic Citation
Journal of Microbiology, vol. 41, no. 4, pp. 306-311
Publication Year
2003
Abstract
In Korea, a Lymantria dispar multinucleocapsid nucleopolyhedrovirus, LdMNPV-NM, was isolated and characterized from dead L. dispar larvae. The polyhedra of LdMNPV-NM were irregularly shaped with a diameter of 1.62±0.33 μm. Numerous virions comprised of the multinucleocapsid were evident in the electron microscopic examination of the polyhedra cross sections. These polyhedra were composed of a major protein of 30 kDa. The restriction enzyme digestion patterns of LdMNPV-NM showed that this isolate had some different fragments from those of the Gypchek® LdMNPV isolate, although their overall profiles were similar. The deduced amino acid sequence of the enhancin gene of LdMNPV-NM showed differences when compared to previously reported enhancin genes of other LdMNPV strains. These results suggested that the LdMNPV-NM isolate from Korea was a new NPV strain and had a new enhancin gene.
Keyword
baculovirusenhancinLdMNPV-NMlymantria dispar
ISSN
1225-8873
Publisher
Microbiological Society Korea
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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