LAMMER kinase homolog, Lkh1, is involved in oxidative-stress response of fission yeast

Abstract

Previously, we reported that the LAMMER kinase homolog, Lkh1, is a negative regulator of filamentous growth and asexual flocculation in the fission yeast, Schizosaccharomyces pombe. Here, we report that the lkh1 + null mutant is sensitive to oxidative stress because of a reduction in the expression of genes for antioxidant enzymes such as catalase (ctt1+) and Cu,Zn-superoxide dismutase (sod1+). Furthermore, the lkh1+ null mutant shows increased levels of intracellular peroxides under conditions of oxidative stress compared with wild-type cells. Interestingly, expression of the gene for the transcription factor Atf1 is reduced in the lkh1+ null mutant under oxidative stress, whereas expression of the transcription factor Pap1 is not. We report the novel finding that Lkh1 is involved in the oxidative-stress response of the fission yeast, S. pombe, and regulates the expression of antioxidant enzymes via the transcription factor Atf1.