Mad1p, a component of the spindle assembly checkpoint in fission yeast, suppresses a novel septation-defective mutant, sun1, in a cell-division cycle

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Title
Mad1p, a component of the spindle assembly checkpoint in fission yeast, suppresses a novel septation-defective mutant, sun1, in a cell-division cycle
Author(s)
I G Kim; D K Rhee; J W Jeong; S C Kim; Mi Sun Won; J H Lee; K W Song; H B Kim
Bibliographic Citation
FEMS Microbiology Letters, vol. 227, no. 2, pp. 183-188
Publication Year
2003
Abstract
To enhance our understanding of the cytokinesis, we have carried out a genetic screen for temperature-sensitive Schizosaccharomyces pombe mutants that show defects in septum formation and cell division. Here we present the isolation and characterization of a new temperature-sensitive mutant, sun1 (septum uncontrolled), which undergoes uncontrolled septation during cell-division cycle at restrictive temperature (37°C). In sun1 mutant, the actin ring and septum are positioned at random locations and angles, and the nuclear division cycle continues. These observations suggest that the sun1 gene product is required for the proper placement of the actin ring as well as precise septation. In a screen for the sun1+ gene to complement the sun1 mutant, we have isolated a mad1+ (mitotic arrest deficient) gene, which encodes a component of the spindle checkpoint in the cell-division cycle. Analysis of crossing the sun1 cell with the mad1+ null mutant indicates that mad1+ suppresses the sun1 mutant defective in controlled septation in a cell-division cycle.
Keyword
Cell-division cycleCytokinesismad1pSchizosaccharomyces pombeSpindle checkpoint
ISSN
0378-1097
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1016/S0378-1097(03)00607-4
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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