An improved method for constructing a full-length enriched cDNA library using small amounts of total RNA as a starting material

Cited 16 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJung Hwa Oh-
dc.contributor.authorYong Sung Kim-
dc.contributor.authorNam-Soon Kim-
dc.date.accessioned2017-04-19T09:00:47Z-
dc.date.available2017-04-19T09:00:47Z-
dc.date.issued2003-
dc.identifier.issnI000-0028-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6396-
dc.description.abstractWe have developed an improved method for constructing a full-length cDNA library using small quantity of material by modifying the original oligo-capping method. In our devised method, total RNAs are used in sequential oligo-capping steps directly without preliminary mRNA purification. Using this method, we constructed full-length cDNA libraries from 100 μg of total RNA. These libraries contained 8 × 105 to 8 × 106 independent clones with average insert sizes of 2.0 kb. Moreover, the number of full-length cDNAs containing the translation initiation codon ATG in the constructed libraries was estimated to 60-70%. In addition, 54% of the known cDNAs had a longer 5′ end than the corresponding genes in the public database. Our results show that the method can be effectively used to construct full-length enriched cDNA libraries, especially, if starting material is limited.-
dc.publisherSpringer-Nature Pub Group-
dc.titleAn improved method for constructing a full-length enriched cDNA library using small amounts of total RNA as a starting material-
dc.title.alternativeAn improved method for constructing a full-length enriched cDNA library using small amounts of total RNA as a starting material-
dc.typeArticle-
dc.citation.titleExperimental and Molecular Medicine-
dc.citation.number6-
dc.citation.endPage590-
dc.citation.startPage586-
dc.citation.volume35-
dc.contributor.affiliatedAuthorJung Hwa Oh-
dc.contributor.affiliatedAuthorYong Sung Kim-
dc.contributor.affiliatedAuthorNam-Soon Kim-
dc.contributor.alternativeName오정화-
dc.contributor.alternativeName김용성-
dc.contributor.alternativeName김남순-
dc.identifier.bibliographicCitationExperimental and Molecular Medicine, vol. 35, no. 6, pp. 586-590-
dc.identifier.doi10.1038/emm.2003.77-
dc.subject.keywordcDNA library-
dc.subject.keywordFull-length-
dc.subject.keywordOligo-capping-
dc.subject.keywordTotal RNA-
dc.subject.localcDNA Library-
dc.subject.localcDNA library-
dc.subject.localFull-length-
dc.subject.localOligo-capping-
dc.subject.localTotal RNA-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.