Costunolide inhibits interleukin-1β expression by down-regulation of AP-1 and MAPK activity in LPS-stimulated RAW 264.7 cells

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dc.contributor.authorJong Soon Kang-
dc.contributor.authorYeo Dae Yoon-
dc.contributor.authorKi Hoon Lee-
dc.contributor.authorSong Kyu Park-
dc.contributor.authorHwan Mook Kim-
dc.date.accessioned2017-04-19T09:00:47Z-
dc.date.available2017-04-19T09:00:47Z-
dc.date.issued2004-
dc.identifier.issn0006291X-
dc.identifier.uri10.1016/j.bbrc.2003.11.109ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/6401-
dc.description.abstractCostunolide, a sesquiterpene lactone isolated from the root of Saussurea lappa Clarke, is known to have a variety of biological activities, including anti-carcinogenic and anti-fungal activities. Here, we demonstrated the inhibitory effect of costunolide on the protein and mRNA expression of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. We also showed that costunolide suppressed the transcriptional activity of the IL-1β promoter. Moreover, costunolide inhibited the activity of AP-1 transcription factor, and the phosphorylation of MAPKs, including SAPK/JNK and p38 MAP kinase. The inhibitory effect of costunolide on AP-1 activity was also confirmed by an electrophoretic mobility shift assay. Additionally, specific inhibitors of SAPK/JNK and p38 MAP kinase, SP600125 and SB203580, also suppressed LPS-induced increase in IL-1β gene expression and AP-1 DNA binding. Taken together, these results demonstrate that costunolide inhibits IL-1β gene expression by blocking the activation of MAPKs and DNA binding of AP-1 in LPS-stimulated RAW 264.7 cells.-
dc.publisherElsevier-
dc.titleCostunolide inhibits interleukin-1β expression by down-regulation of AP-1 and MAPK activity in LPS-stimulated RAW 264.7 cells-
dc.title.alternativeCostunolide inhibits interleukin-1β expression by down-regulation of AP-1 and MAPK activity in LPS-stimulated RAW 264.7 cells-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number1-
dc.citation.endPage177-
dc.citation.startPage171-
dc.citation.volume313-
dc.contributor.affiliatedAuthorJong Soon Kang-
dc.contributor.affiliatedAuthorKi Hoon Lee-
dc.contributor.alternativeName강종순-
dc.contributor.alternativeName윤여대-
dc.contributor.alternativeName이기훈-
dc.contributor.alternativeName박성규-
dc.contributor.alternativeName김환묵-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 313, no. 1, pp. 171-177-
dc.identifier.doi10.1016/j.bbrc.2003.11.109-
dc.subject.keywordAP-1-
dc.subject.keywordCostunolide-
dc.subject.keywordInflammation-
dc.subject.keywordInterleukin-1β-
dc.subject.keywordMAP kinase-
dc.subject.localAP-1-
dc.subject.localCostunolide-
dc.subject.localInflammation-
dc.subject.localInterleukin-1β-
dc.subject.localMAP kinase-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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