Heyneanol A induces apoptosis via cytochrome c release and caspase activation in human leukemic U937 cells

Cited 30 time in scopus
Metadata Downloads
Title
Heyneanol A induces apoptosis via cytochrome c release and caspase activation in human leukemic U937 cells
Author(s)
E O Lee; Byoung-Mog Kwon; G Y Song; C H Chae; H M Kim; I S Shim; K S Ahn; S H Kim
Bibliographic Citation
Life Sciences, vol. 74, no. 18, pp. 2313-2326
Publication Year
2004
Abstract
Heyneanol A, a tetramer of resveratrol, is isolated from the roots of Vitis amurensis by cytotoxicity based fractionation. In this study, the mechanism of apoptosis by heyneanol A was evaluated in human leukemic U937 cells. Heyneanol A (IC50 = 6.6 μM at 24 h) exhibited stronger cytotoxic effect than resveratrol (IC50 = 100 μM at 24 h) by 15-fold on human leukemic U937 cells by XTT assay. Apoptotic bodies were observed in U937 cells treated with 6 μM of heyneanol A by TUNEL assay. Heyneanol A effectively increased the portion of sub-G1 DNA content in a time- and concentration-dependent manner by flow cytometric analysis. Heyneanol A also induced cytochrome c release from mitochondria into the cytosol and subsequent caspase activation involving caspase 9 and 3 to cleave PARP. However, it did not affect the expressions of Bax and Bcl-2 by western blotting. It was confirmed that the activation of caspase 8, 9 and 3 and the cleavage of PARP by heyneanol A were completely blocked by adding Z-VAD-FMK, a caspase inhibitor. These findings suggest that heyneanol A has anti-tumor activity, which may be mediated by apoptosis caused by cytochrome c release and caspase activation in human leukemic U937 cells.
Keyword
ApoptosisCaspaseCytochrome cHeyneanol APARPU937 cellsVitis amurensisZ-VAD-FMK
ISSN
0024-3205
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.lfs.2003.10.004
Type
Article
Appears in Collections:
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.