Effect of sealed and vented gaseous microenvironments on the hyperhydricity of potato shoots in vitro

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Title
Effect of sealed and vented gaseous microenvironments on the hyperhydricity of potato shoots in vitro
Author(s)
S W Park; Jae Heung JeonHyun Soon Kim; Y M Park; C Aswath; Hyouk Joung
Bibliographic Citation
Scientia Horticulturae, vol. 99, no. 2, pp. 199-205
Publication Year
2004
Abstract
Different types of ventilation of the culture vessel with and without the ethylene inhibitor KMnO4 in the culture media were investigated in terms of their effect on hyperhydricity of in vitro cultured potato shoots. The shoots grown in a completely sealed vessel (CSV) were severely vitrified, but the shoots cultured in a gas permeable vessel (GPV) showed normal shoot proliferation. Dry weight percentage and chlorophyll content were lower in the vitrified shoots than in the normal shoots. The levels of ethylene and CO 2 were significantly higher in the CSV, but little ethylene was detected in the GPV system. When an ethylene scrubber (perlite treated with KMnO4) was applied to the CSV, the shoots exhibited normal growth without any sign of vitrification. In addition, no ethylene was found in CSV with the ethylene scrubber (CSVE). These results indicate that hyperhydricity is related to the ethylene accumulated in the culture vessel during the in vitro culture of potato shoots. It is suggested to use CSVE to reduce the ethylene level and overcome hyperhydricity.
Keyword
ethylene scrubberhyperhydricityin vitro cultureKMnO4solanum tuberosum
ISSN
0304-4238
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0304-4238(03)00097-9
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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