In vivo neutralization of hepatitis B virus infection by an anti-preS1 humanized antibody in chimpanzees

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Title
In vivo neutralization of hepatitis B virus infection by an anti-preS1 humanized antibody in chimpanzees
Author(s)
Hyo Jeong Hong; Chun Jeih Ryu; Hyang Suk Hur; S H Kim; H K Oh; M S Oh; S Y Park
Bibliographic Citation
Virology, vol. 318, no. 1, pp. 134-141
Publication Year
2004
Abstract
Previously, we generated a murine monoclonal antibody (mAb), KR127, that recognizes amino acids (aa) 37-45 of the preS1 of hepatitis B virus (HBV). In this study, we have constructed a humanized version of KR127 and evaluated its HBV-neutralizing activity in chimpanzees. A study chimpanzee was given a single intravenous dose of the humanized antibody, followed by intravenous challenge with adr subtype of wild type HBV, while a control chimpanzee was only challenged with the virus. The result showed that the study chimpanzee did not develop HBV infection during 1 year, while the control chimpanzee was infected, indicating that the humanized antibody exhibited in vivo virus-neutralizing activity and thus protected the chimpanzee from HBV infection. In addition, the humanized antibody bound to the preS1 of all subtypes of HBV. We first demonstrate that an anti-preS1 mAb can neutralize HBV infection in vivo. This humanized antibody will be useful for the immunoprophylaxis of HBV infection.
Keyword
ChimpanzeeHepatitis B virusHumanized antibodyNeutralizationpreS1
ISSN
0042-6822
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.virol.2003.09.014
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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