Neutralizing human monoclonal antibodies to hepatitis A virus recovered by phage display

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Title
Neutralizing human monoclonal antibodies to hepatitis A virus recovered by phage display
Author(s)
Sang Jick Kim; Myeong Hee Jang; J T Stapleton; Sun Ok Yoon; K S Kim; E S Jeon; Hyo Jeong Hong
Bibliographic Citation
Virology, vol. 318, no. 2, pp. 598-607
Publication Year
2004
Abstract
Four human monoclonal antibodies (MAbs) to hepatitis A virus (HAV) were isolated from a phage-displayed antibody library constructed from the peripheral blood lymphocytes (PBLs) of HAV-immune donors. The four MAbs showed differences in their affinity: two (HA6, HA9) of them were dominant after four rounds of panning, and showed higher affinity than the other two (HA1, HA12). All four MAbs showed HAV-neutralizing activity in radioimmunofocus inhibition assay and their neutralizing activity was positively correlated with their affinities. Analysis of their epitope specificity by cross-competition binding assays suggested that HA6 and HA9 recognize extensively overlapping epitopes, which overlap with those of HA1 and HA12, although HA1 and HA12 recognize distinct epitopes. In addition, competition assays with known neutralizing murine MAbs suggested that the epitopes of four human MAbs extensively overlap with those of B5B3 and K34C8 which are distinct but reside within the single, immunodominant neutralization site on the HAV capsid. The human MAbs (HA6 and HA9) with highest affinity may be useful in the immunoprophylaxis of HAV infection.
Keyword
FabHAVHuman antibody libraryNeutralization epitopePhage display
ISSN
0042-6822
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.virol.2003.10.014
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
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